摘要
Abstract
Objective To investigate the effect of acupotomy lysis on the expressions of cartilage matrix metalloproteinase-3 (MMP-3) and typeⅡ collagen (Col-Ⅱ) in rabbits with knee osteoarthritis (KOA).Methods Of 40 6-month-old healthy New Zealand rabbits of clean grade, 10 were randomly selected as a blank group and the other 30 were used for model making. A KOA model was made by joint immobilization. After successful model making, the 30 rabbits were randomized into model, acupotomy and electroacupuncture groups, 10 rabbits each. The acupotomy group received acupotomy lysis of knee lateral collateral ligaments and patellar ligament by longitudinal loosening and transverse stripping, once a week for a total of three times. The electroacupuncture group was given electroacupuncture at points Yinlingquan, Yanglingquan, media Xiyan and Dubi, at a frequency of 2-100 Hz and an intensity of 3 mA, 20 min thrice a week for a total of three weeks. Morphological changes in the cartilage were observed by optical microscopy. The expressions of Col-Ⅱ and MMP-3 in knee cartilage were determined by Western blotting. Results HE staining showed different degrees of hyperplasia and edema of knee joint synovium in the model group of rabbits. Optical microscopy showed an obvious degenerative change in the articular cartilage in the model group. Injury to the cartilage tissue was reduced and had a tendency to be repaired in the electroacupuncture and acupotomy groups. Knee cartilage destruction was significantly reduced in the acupotomy group of rabbits. Col-Ⅱ expression was higher in the acupotomy group than in the model group (P<0.01). There was a statistically significant difference in Col-Ⅱ expression between the acupotomy and electro- acupuncture groups (P<0.05). Col-Ⅱ expression was low in the model group and there was a statistically significant difference compared with the other groups (P<0.05). MMP-3 expression was significantly higher in the model group than in the blank group (P<0.01). MMP-3 expression was lower in the acupotomy group than in the blank group (P<0.01) but there was no statistically significant difference (P>0.05). There was a statistically significant difference in MMP-3 expression between the acupotomy group and the model or electroacupuncture group (P<0.01,P<0.05).Conclusions Acupotomy lysis can reduce cartilage MMP-3 level and increase Col-Ⅱ expression to prevent cartilage degeneration and produce a therapeutic effect in the treatment of KOA rabbits.关键词
针刺疗法/小针刀/电针/骨关节炎,膝关节/兔/基质金属蛋白酶-3/二型胶原Key words
Acupuncture therapy/Small needle knife/Electroacupuncture/Osteoarthritis, knee joint/Rabbits/Matrix metalloproteinase-3/TypeⅡ collagen分类
医药卫生
