西南林业大学学报2016，Vol.36Issue(6)：1-7,7.DOI:10.11929/j.issn.2095-1914.2016.06.001
霍山石斛拟原球茎生长及植株再生过程中微观结构与元素的变化
Changes in Micro-structure and Elements of Protocorm Like Bodies from Dendrobium huoshanense During the Development and Plantlet Regeneration
摘要
Abstract
In order to get better understanding of the growth and officinal components during the development of protocorm like bodies ( PLBs) from Dendrobium huoshanense and plantlet regeneration, changes in micro-struc-ture and micro-area elements were analyzed by means of SEM and EDS. Cells which have the ability to divide from the surface of explants formed embryogenic callus in tissue culture when conditions were suitable. Embryogenic cells grew into PLBs and secondary PLBs after constant division. PLBs showed bipolarity gradually and produced buds and roots to form complete plants. After 30 days, root hair or velamina were not found in roots. Stems were charac-terized by several features such as surface longitudinal depressions, round cross sections with wavy edges, scattered external ductile finite vascular bundle and mucinous cells contents. Quadrilateral stomata only distributed in the lower epidermis of isobilateral leaves and the guard cells were like kidneys. And more Na, S, K, Al, Si and Fe were in PLBs, then the contents of P, Cl, Mn and Ca increased in differentiated PLBs, and the contents of Na, S, K increased in the complete plants while other elements were less or not changed significantly.关键词
霍山石斛/拟原球茎/植株再生/微观结构/元素Key words
Dendrobium huoshanense/Protocorm Like Bodies (PLBs)/plantlet regeneration/micro-struc-ture/element分类
农业科技引用本文复制引用
杨静,何芳,王纪,方炎明,戴斌..霍山石斛拟原球茎生长及植株再生过程中微观结构与元素的变化[J].西南林业大学学报,2016,36(6):1-7,7.基金项目
江苏省林业三新工程项目(lysx[2013]07)资助 （lysx[2013]07）
国家自然科学基金(31200233)资助. （31200233）
