西南林业大学学报2016，Vol.36Issue(6)：41-46,6.DOI:10.11929/j.issn.2095-1914.2016.06.007
杂种白杨717-1B4组培技术研究
Micropropagation Techniques of Hybrid Aspen Clone 717-1B4
摘要
Abstract
In order to maintain the maternal excellent features in offspring and refrain from difficult rooting in vegetative propagation of hybrid aspen, established a juvenility and container rooting-hardening micropropagation system in the present work. The result showed that explants taken from the sprouting between root-shoot junctions of maternal plants, sterilized by soaked 1. 0% effective chlorine of sodium dichloroisocyanurate ( NaDCC) solution for 25 min, cultured on MS medium, 75. 91% of them regenerated juvenile shoots. The explants regenerated more than 3 juvenile shoots were selected and sub-cultured on the same fresh medium in each 30 days, each explant produced 12. 2 shoots on average. The shoots from explants multiplicated one time before did container rooting and hardening. The shoots increased 3. 73 times on MS medium containing 6-BA 0. 5 mg/L and NAA 0. 2 mg/L, respectively. The rooting rate reached the highest at 98. 89% when IBA was 2. 0 mg/L in rooting medium. The container plantlets hardened in reversion and acclimatization by shifting and opening gradually the tissue culture bottles. The container rooted aspen plants survived at 92. 5% in the acclimatization stage.关键词
杂种/白杨717-1B4/组织培养/不定芽/生根/炼苗Key words
hybrid/aspen 717-1B4/micropropagation/adventitious bud/rooting/plantlets hardening分类
农业科技引用本文复制引用
郑瑞丰,赵博文,叶融,张文彪,王华芳..杂种白杨717-1B4组培技术研究[J].西南林业大学学报,2016,36(6):41-46,6.基金项目
国家自然科学基金(3370598) （3370598）
国家自然科学基金(J1103516). （J1103516）
