西南林业大学学报2016，Vol.36Issue(6)：112-116,5.DOI:10.11929/j.issn.2095-1914.2016.06.018
管氏肿腿蜂寄生对黄粉甲硫氧还蛋白基因转录的影响
Transcriptional Regulation of Tenebrio molitor Thioredoxin Gene by Scleroderma guani Parasitization
摘要
Abstract
Thioredoxin proteins maintain the stability of the redox state and are involved in essential antioxida-tion and oxidation reduction process regulation in insects. In order to reveal the functions of the thioredoxin proteins gene and its transcription regulated by Scleroderma guani parasitization, this study cloned the full-length cDNA se-quence of thioredoxin gene from Tenebrio molitor by rapid amplification of cDNA ends strategy. The full-length cDNA sequence of thioredoxin gene was 531 bp, and contained a 318 bp open reading frame encoding 106 amino acid res-idues, with 3′and 5′untranslated regions of 95 bp and 119 bp, respectively. Its predicted molecular weight and isoelectric point were 11. 97 kDa and 4. 22, respectively. Its deduced amino acid sequence contained a highly con-served CGPC active site at the N-terminus. Multiple sequence alignment analysis showed that thioredoxin of T. molitor shared over 60% amino acid identities with those of other insects. qPCR analysis showed that thioredoxin gene was expressed at different developmental stages of T. molitor with the highest expression in the pupa and the lowest in the adult. This gene highly expressed in fat body of pupae. After 6 h parasitization by S. guani, the relative expression of thioredoxin gene was obviously up-regulated.关键词
黄粉甲/硫氧还蛋白/基因克隆/序列分析/管氏肿腿蜂Key words
Tenebrio molitor/thioredoxin/gene cloning/sequence analysis/Scleroderma guani分类
农业科技引用本文复制引用
黄镜梅,刘乃勇,张祖兵,杨斌,朱家颖..管氏肿腿蜂寄生对黄粉甲硫氧还蛋白基因转录的影响[J].西南林业大学学报,2016,36(6):112-116,5.基金项目
国家自然科学基金项目(31260449)资助 （31260449）
云南省林学一级学科博士点建设项目(林学学位授权学科项目经费)资助 （林学学位授权学科项目经费）
云南省中青年学术与技术带头人后备人才项目(2013HB077)资助 （2013HB077）
云南省高校森林有害生物科技创新团队项目资助. （）
