中国病理生理杂志2016，Vol.32Issue(11)：1979-1983,5.DOI:10.3969/j.issn.1000-4718.2016.11.010
miR-497与肾癌预后的关系及其对肾癌786-0细胞增殖、凋亡和侵袭的影响
Association of miR-497 and prognosis in patients with renal cell carcino-ma and its effects on proliferation, apoptosis and invasion of human renal cell carcinoma cell line 786-0
张旖骁 1吴斌1
作者信息
- 1. 中国医科大学附属盛京医院第一泌尿外科，辽宁 沈阳110004
- 折叠
摘要
Abstract
AIM:To investigate the association of microRNA-497 (miR-497) and prognosis in the patients with re-nal cell carcinoma (RCC) and its effects on the proliferation, apoptosis and invasion of human RCC cell line 786-0. METH-ODS:Paired RCC and adjacent non-tumor tissue specimens were surgically collected from 80 patients who were diagnosed with primary RCC between 2011 and 2015. The expression of miR-497 in the paired RCC and adjacent non-tumor tissue specimens was detected by real-time PCR. Recurrence-free survival was estimated using the Kaplan-Meier method and compared using the log-rank test. The 786-0 cells were transfected with miR-497 mimics or scramble control miRNA. The proliferation, apoptosis and in-vasion abilities of the transfected cells were assessed by MTT assay, Trypan blue exclusion, flow cytometry and Transwell cham-ber experiment. The protein expression of miR-497-targeted gene cyclin D1 in the transfected cells was quantified by Western blotting. RESULTS:miR-497 was down-regulated in the RCC specimens compared with the adjacent tissues. miR-497 was down-regulated in the RCC 786-0 cells compared with the HK-2 cells. By the end of the study, 74 cases were followed up. The follow-up rate was 92. 5%. Median follow-up was 29 months (ranging from 2 months to 48 months). The 3-year recurrence-free survival rates of the patients with high and low miR-497 expression were 71. 2% and 40. 1%, respectively. Over-expression of miR-497 resulted in significant suppression effect on RCC cell proliferation, invasion and the expression of cyclin D1. CON-CLUSION:Low expression of miR-497 was correlated with poor prognosis in the RCC patients. miR-497 inhibits proliferation and invasion of RCC 786-0 cells and its mechanism is associated with the down-regulation of cyclin D1.关键词
肾细胞癌/微小RNA-497/细胞增殖/预后Key words
Renal cell carcinoma/MicroRNA-497/Cell proliferation/Prognosis分类
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张旖骁,吴斌..miR-497与肾癌预后的关系及其对肾癌786-0细胞增殖、凋亡和侵袭的影响[J].中国病理生理杂志,2016,32(11):1979-1983,5.
