中国病理生理杂志2016，Vol.32Issue(11)：1996-2002,7.DOI:10.3969/j.issn.1000-4718.2016.11.013
星形胶质细胞的缝隙连接蛋白43参与冷冻伤后脑水肿的形成∗
Astrocytic connexin 43 is involved in development of cryoinjury-induced cerebral edema
摘要
Abstract
AIM: To discuss the change of astrocytic connexin 43 (Cx43) expression in cerebral edema and the potential roles of Cx43 in the development of cerebral edema. METHODS:Cryoinjury-induced cerebral edema model was established in the right parietal lobe cortex in the rats. The animals were divided into sham group, cryoinjury-induced cerebral edema model group, and cryoinjury-induced cerebral edema model injected with carbenoxolone ( or octanol ) group. Dry and wet weight method was used to measure the content of brain water. Methanamide method was used to evalu-ate the permeability of blood-brain barrier ( BBB) after cryoinjury. HE staining showed the physiological changes in injured brain cortex. The protein expression of Cx43 was detected by Western blot and immunohistochemical staining. RESULTS:The brain water content in the injured cortex of rats was increased and cerebral edema developed to the peak at 24 h after cryoinjury. The permeability of BBB was increased in the injured cortex of the rats, and the area where BBB was damaged was larger than injured area. HE staining showed that many cells were dead in the central area of cryoinjury, and at the same time cerebral edema was appeared obviously in the surrounding area of injured brain cortex. The protein expression of Cx43 was up-regulated in the surrounding area of injured brain cortex, while Cx43 expression decreased in the injured cor-tex at different periods after cryoinjury. Both the content of brain water in the injured cortex and the permeability of BBB at 24 h after cryoinjury were reduced after injecting carbenoxolone or octanol intraperitoneally to block Cx43. CONCLU-SION:The cerebral edema up-regulates the expression of Cx43 and also strengthens its function. Once Cx43 is blocked, the degree of cerebral edema in rats is attenuated.关键词
缝隙连接蛋白43/星形胶质细胞/脑水肿/缝隙连接/冷冻伤Key words
Connexin 43/Astrocytes/Cerebral edema/Gap junction/Cryoinjury分类
医药卫生引用本文复制引用
章科娜,丁悦敏,杜月光,龚婉,张雄..星形胶质细胞的缝隙连接蛋白43参与冷冻伤后脑水肿的形成∗[J].中国病理生理杂志,2016,32(11):1996-2002,7.基金项目
浙江省教育厅科研项目(No. Y201120080) （No. Y201120080）
浙江中医药大学校级科研基金(No.2014ZY02) （No.2014ZY02）
