中国病理生理杂志2016，Vol.32Issue(11)：2003-2009,7.DOI:10.3969/j.issn.1000-4718.2016.11.014
黄芪甲苷与人参皂苷Rg1配伍对PC12细胞氧糖剥夺复糖复氧后细胞自噬和PI3 K信号通路的影响∗
Effects of astragaloside IV combined with ginsenoside Rg1 on autophagy and PI3 K signaling pathways of PC12 cells induced by oxygen glucose deprivation/reoxygenation
摘要
Abstract
[ ABSTRACT] AIM:To probe the effect and the mechanism of astragaloside IV and ginsenoside Rg1 on autophagy of PC12 cells induced by oxygen glucose deprivation/reoxygenation ( OGD/R) . METHODS:The autophagy injury model of PC12 cells induced by OGD/R was established( PC12 cells were exposed to 2 h of OGD followed by 24 h of reoxygen-ation) . The effects of astragaloside IV combined with ginsenoside Rg1 on autophagy of PC12 cells were observed, and the mechanism was studied through PI3KⅠ/Akt/mTOR and PI3KⅢ/becline-1/Bcl-2 signaling pathways. RESULTS:After OGD/R, LC3-Ⅱ/LC3-Ⅰin PC12 cells was increased. Astragaloside IV, ginsenoside Rg1 and astragaloside IV combined with ginsenoside Rg1 restrained the increase in LC3-Ⅱ/LC3-Ⅰ, the effect of the combination was greater than using the drug alone. Ginsenoside Rg1, astragaloside IV combined with ginsenoside Rg1 up-regulated the phosphorylation level of PI3K Ⅰ, Akt and mTOR. The effects of the combination were stronger than those of using the drug alone. Astragaloside IV, astragaloside IV combined with ginsenoside Rg1 inhibited the protein expression of PI3KⅢand becline-1, the effects of the combination were better than those of single astragaloside IV and single ginsenoside Rg1. Meanwhile, the combina-tion treatment increased Bcl-2 protein expression. CONCLUSION:The autophagy of PC12 cells induced by OGD/R is in-hibited by astragaloside IV and ginsenoside Rg1. Furthermore, astragaloside IV combined with ginsenoside Rg1 plays syn-ergitic inhibition on autophagy, the mechanism may be related to PI3KⅠ/Akt/mTOR and PI3KⅢ/becline-1/Bcl-2 signa-ling pathways.关键词
黄芪甲苷/人参皂苷Rg1/PC12 细胞/自噬/PI3K Ⅰ/Akt/mTOR信号通路/PI3K Ⅲ/beclin-1/Bcl-2信号通路Key words
Astragaloside IV/Ginsenoside Rg1/PC12 cells/Autophagy/PI3K Ⅰ/Akt/mTOR signaling pathway/PI3K Ⅲ/becline-1/Bcl-2 signaling pathway分类
医药卫生引用本文复制引用
丁煌,李静娴,杨筱倩,唐标,刘晓丹,唐映红,邓常清,黄小平..黄芪甲苷与人参皂苷Rg1配伍对PC12细胞氧糖剥夺复糖复氧后细胞自噬和PI3 K信号通路的影响∗[J].中国病理生理杂志,2016,32(11):2003-2009,7.基金项目
湖南省科技厅一般项目(No.2014SK3001) （No.2014SK3001）
中西医结合防治心脑血管疾病的相关基础研究 （）
“中医药防治心脑血管疾病基础研究”湖南省自然科学创新群体基金 （）
