中国病理生理杂志2016，Vol.32Issue(11)：2091-2094,4.DOI:10.3969/j.issn.1000-4718.2016.11.030
槐榆煎剂对肛肠手术后大鼠血清中TNF-α、IL-6和IL-10含量的影响
Effects of Huaiyu decoctum on serum concentrations of TNF-α, IL-6 and IL-10 in rats after anorectal operation
刘雨燕 1许向前 1罗文昭1
作者信息
- 1. 河南省中医院肛肠科，河南 郑州450002
- 折叠
摘要
Abstract
AIM: To investigate the effects of Huaiyu decoctum on the serum concentrations of TNF-α, IL-6 and IL-10 in rats after anorectal operation. METHODS: Sprague-Dawley rats ( n =40 ) were randomly divided into 4 groups:normal group, model group, low-dose Huaiyu decoctum group and high-dose Huaiyu decoctum group. The concen-trations of TNF-α, IL-6 and IL-10 in the rat serum were measured by ELISA. The pathologic changes of the anorectal tis-sues were observed under microscope with HE staining. The protein expression of ICAM-1, VCAM-1 and NF-κB was deter-mined by Western blotting. RESULTS:After Huaiyu decoctum administration, TNF-αand IL-6 concentrations in the ser-um were significantly decreased, and IL-10 concentration was increased as compared with model group. Moreover, Huaiyu decoctum markedly attenuated edema and hyperemia in the rats after anorectal operation. The protein expression of intercel-lular adhesion molecule-1 ( ICAM-1 ) , vascular cell adhesion molecule-1 ( VCAM-1 ) and NF-κB in the anorectal tissues was obviously inhibited by Huaiyu decoctum treatment. CONCLUSION:Huaiyu decoctum improves the recovery of ano-rectal tissues after operation by decreasing the serum concentrations of TNF-α, IL-6 and IL-10, and reducing the protein expression of ICAM-1, VCAM-1 and NF-κB in the anorectal tissues.关键词
槐榆煎剂/肛肠手术/炎性细胞因子Key words
Huaiyu decoctum/Anorectal operation/Inflammatory cytokines分类
医药卫生引用本文复制引用
刘雨燕,许向前,罗文昭..槐榆煎剂对肛肠手术后大鼠血清中TNF-α、IL-6和IL-10含量的影响[J].中国病理生理杂志,2016,32(11):2091-2094,4.
