中国免疫学杂志2016，Vol.32Issue(11)：1593-1597,5.DOI:10.3969/j.issn.1000-484X.2016.11.006
激活核受体PXR促进小鼠GM-CSF来源的树突状细胞的分化
Activation of PXR promotes differentiation of mouse GM-CSF derived dendritic cells
摘要
Abstract
Objective:To investigate the influence of Pregnane X Receptor ( PXR) agonist pregnane-16α-carbonitrile ( PCN) on mouse GM-CSF derived dendritic cell ( DC) differentiation.Methods:Mouse DC differentiation was induced by GM-CSF and IL-4 in vitro,and treated with PCN.The proportion of CD11c+DC was measured by flow cytometry;the production of Interferon-γ(IFN-γ), Interleukin-1 (IL-1),Interleukin-2 (IL-2) and Interleukin-12 (IL-12) of DC were analyzed by ELISA and flow cytometry;Real-time RT-RCR was used to detect the expression of genes involved in the signaling pathway which regulated DC differentiation .Results:Mouse DC was successfully induced by GM-CSF and IL-4 in vitro.Treatment of PCN significantly increased the percentage of CD 11c+DC and the protein levels of IFN-γand IL-2 produced by DC were notably enhanced.Real-time RT-RCR results indicated that the expression of Notch and Wnt signaling pathway related genes HES 1,WISP1 and WISP2 were up-regulated in PCN treatment group when compared to control group.Conclusion: The PXR agonist PCN can promote the differentiation of mouse GM-CSF derived DC and increased the production of IFN-γand IL-2 by DC,and Notch and Wnt signaling pathway may play a pivotal role in this process.关键词
树突状细胞/分化/Pregnane X Receptor/Notch信号通路/Wnt信号通路Key words
Dendritic cells/Differentiation/Pregnane X Receptor/Notch signaling pathway/Wnt signaling pathway分类
医药卫生引用本文复制引用
邱怀娜,查贺飞,曲佳乐,王梅,李璐,齐艳伟,黄俊,杨权..激活核受体PXR促进小鼠GM-CSF来源的树突状细胞的分化[J].中国免疫学杂志,2016,32(11):1593-1597,5.基金项目
本文受广州医科大学科学科研项目资助（2015C01）。 （）
