中国免疫学杂志2016，Vol.32Issue(11)：1607-1609,3.DOI:10.3969/j.issn.1000-484X.2016.11.009
小鼠脾脏淋巴细胞体外培养前后细胞表面标记物 CD3、CD4、CD44、CD62 L的变化
Changes of cell surfca e markers CD3,CD4,CD44,CD62L on mouse spleen lym-phoycets fater cultuer in vitro
李艳春 1赵丽娜 1张献宇 2乔红梅1
作者信息
- 1. 吉林大学第一医院小儿呼吸一科，长春 130021
- 2. 吉林大学中日联谊医院甲状腺外科，长春 130033
- 折叠
摘要
Abstract
Objective:To study the changes of cell surface markers CD 3,CD4,CD44 and CD62L on mouse spleen lymphocytes after culture in vitro.Methods:Spleen cells were separated from BALB/c mice and asthmatic mice and lymphocytes were isolated by using lymphocyte separation liquid.We cultured lymphocytes with RPMI 1640 medium(10%FBS) in cell incubator for 3 days.Then the four cell surface markers were detected by using flow cytometry.Results:In normal mice ,19.09%of lymphocytes stained positively for CD3 and CD4,among these cells 98.61% was CD44+T cell and 68.71% was CD62L+T cell before the culture.After culture with ConA for 3 days,CD3+CD4+and CD3+CD4+CD44+T cell population was down regulated to 8.96% and 71.82%,respectively.While CD62L almost disappeared, accounting for 11.27% only.For asthmatic mice, CD3+CD4+T cell accounted for 20.33% in total lymphocytes,among which 97.72%was CD44+and 75.74%was CD62L+before the culture.After culture,CD3+CD4+T cell accounted for 7.2%,among which CD44+only 58.21%and CD62L+only 2.77%.Conclusion:In both normal and asthmatic mice ,CD3,CD4, CD44 and CD62L on spleen lymphocytes were down regulated after culture in vitro regardless with or without stimulating factors , especially CD62L,almost disappeared.This should be given full consideration when these markers are detected after cell culture .关键词
表面标记物/小鼠/淋巴细胞Key words
Cell surface marker/Mouse/Lymphocyte分类
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李艳春,赵丽娜,张献宇,乔红梅..小鼠脾脏淋巴细胞体外培养前后细胞表面标记物 CD3、CD4、CD44、CD62 L的变化[J].中国免疫学杂志,2016,32(11):1607-1609,3.
