中国免疫学杂志2016，Vol.32Issue(11)：1649-1652,4.DOI:10.3969/j.issn.1000-484X.2016.11.018
IL-23在变应性鼻炎小鼠模型中的作用及机制的研究
Effect of IL-23 in a model of allergic rhinitis and its mechanism
摘要
Abstract
Objective:To explore the effect of IL-23 in the treatment of allergic rhinitis in mice.Methods:A model of allergic rhinitis was established with Ovalbumin (OVA),and then the mice were treated with anti-IL-23p19.The nasal rubs were counted.HE staining was performed and the number of total inflammatory cells and eosinophils was counted in the nasal lavage .The content of IL-4, IFN-γand IL-17A were measured by ELISA.The amount of FOXP3 in the local mucosa was measured by Real-time PCR method.Results:Compared to IgG control group,after the treatment of anti-IL-23p19,the nasal rubs were decreased (P<0.05). Compared to PBS group ,the total inflammatory cells in the nasal mucosa and NLF eosinophils showed significant difference for the OVA group(P<0.01 and P<0.05 respectively),but after the anti-IL-23p19 treatment,they were significantly decreased (P<0.05).IFN-γwas not different among different groups ( P>0.05 ).But after the anti-IL23p19 treatment, the IL-4 and IL-17A production was significantly decreased (P<0.05).Moreover,after the anti-IL23p19 treatment,the amount of local FOXP3 was significantly enhanced (P<0.05).Conclusion: Anti-IL-23p19 treatment has a therapeutic effect on the allergic rhinitis of mice partly by limiting Th 17 response and enhancing T regulatory response.关键词
IL-23/变应性鼻炎/IL-17AKey words
IL-23/Allergic rhinitis/IL-17A分类
医药卫生引用本文复制引用
刘阳,余海静,刘争..IL-23在变应性鼻炎小鼠模型中的作用及机制的研究[J].中国免疫学杂志,2016,32(11):1649-1652,4.基金项目
本文为国家自然科学基金项目（81300812）。 （）
