中国全科医学2016，Vol.19Issue(32)：3961-3965,5.DOI:10.3969/j.issn.1007-9572.2016.32.013
甲状腺乳头状癌组织中 Smad4基因表达水平及其意义研究
Expression and Significance of Smad4 Gene in Papillary Thyroid Cancer
摘要
Abstract
Objective To explore the expression and significance of Smad4 gene in papillary thyroid cancer ( PTC) . Methods From January to August in 2013, 20 cases of PTC admitted to Department of Tumor Surgery of Affiliated Hospital of Taizhou University were enrolled as the PTC group, and 20 patients with nodular goiter accepted surgery in the same period were enrolled as the control group.Surgically resected tissue of PTC in the PTC group and normal thyroid tissue adjacent to nodular goiter in the control group were collected. The positive expression of Smad4 protein in two groups was observed using immunohistochemistry , the expression level of Smad4 protein in two groups was detected using Western blotting and the expression level of Smad4 mRNA using real time-PCR.Results The positive expression rate of Smad4 protein in PTC group was 35.0%(7/20), which was lower than 90.0% (18/20) in the control group (χ2 =10.667, P=0.001).There was no statistically significant difference in the positive expression rate of Smad4 protein in the PTC patients among gender, age, tumor diameter, lymph node metastases and clinical stage of PTC ( P >0.05 ) . The expression of Smad4 protein in PTC group was ( 0.352 ±0.012), which was lower than that in control group (0.722 ±0.007) (t=46.130, P<0.001).Smad4 gene expressed in both PTC and control group, the expression level of Smad4 mRNA in PTC tissue was (0.348 ±0.006) time as much as control group.Conclusion The expression of Smad4 gene was down -regulated in PTC. The inhibition of TGF-β/Smads signal pathway caused by the loss and down-regulation of Smad4 gene expression might be the possible mechanisms of the occurrence and development of PTC.关键词
甲状腺肿瘤/癌, 乳头状/Smad4基因/信号传导Key words
Thyroid neoplasms/Carcinoma, papillary/Smad4 gene/Signal transduction分类
医药卫生引用本文复制引用
黄盈瑞,陈洁,周玲玲,梁勇..甲状腺乳头状癌组织中 Smad4基因表达水平及其意义研究[J].中国全科医学,2016,19(32):3961-3965,5.基金项目
2014年浙江省自然科学基金资助项目(LY14H160036）；2014年台州学院青年基金资助项目(2014QN028） （）
