中国全科医学2016，Vol.19Issue(33)：4067-4072,6.DOI:10.3969/j.issn.1007-9572.2016.33.009
氧化苦参碱对高脂饮食诱导胰岛素抵抗载脂蛋白 E基因敲除小鼠肝脏胆固醇代谢调控基因的影响
Effect of Oxymatrine on Hepatic Cholesterol Metabolism Regulated Genes in Insulin Resistance ApoE -/- Mice Induced by High Fat Diet
摘要
Abstract
Objective To investigate the effect of oxymatrine on genes regulated hepatic cholesterol metabolism in ApoE - / - mice induced by high fat diet. Methods From April 2014 to January 2015,seventeen SPF grade C57BL/ 6J mice were selected as normal control(NC)group and fed on the basal diet. Sixty - eight SPF grade ApoE - / - mice fed on high fat diet. After 16 weeks of feeding,according to the random number table method,ApoE - / - mice were averagely divided into insulin resistance model group(IR group),low oxymatrine dose group(OXYL group),middle oxymatrine dose group(OXYM group)and high oxymatrine dose group(OXYH group). There were seventeen mice in each group. In OXYL group,OXYM group and OXYH group,25 mg·kg - 1 ·d - 1 ,50 mg·kg - 1 ·d - 1 and 100 mg·kg - 1 ·d - 1 oxymatrine were given by continuous oral gavage for 8 weeks,and all groups were given equal volume of pure water. Glucose infusion rate(GIR),blood glucose(FBG)level,serum leves of total cholesterol( TC),three glycerol( TG),low density lipoprotein cholesterol( LDL-C),high density lipoprotein cholesterol(HDL-C),fasting insulin( FINS)level in each group of mice were measured,and the insulin resistance index (HOMA - IR)was calculated,and the TC level of liver tissue was detected. The expression of SREBP-2,SCAP,INSIG2, HMGCR and LDLr mRNA and its protein were detected using RT - qPCR and western - blotting methods in mice liver tissue. Results The FBG level,serum levels of TC,TG,LDL-C and HDL-C in IR group was significantly higher than NC group (P < 0. 05). The serum levels of TC,TG,LDL-C and HDL-C in OXYL group was significantly lower than IR group( P <0. 05). The FBG level,serum levels of TC,TG,LDL-C and HDL-C of in OXYM and OXYH group were significantly lower than IR group(P < 0. 05). GIR in IR group was lower than NC group(P < 0. 05);GIR in OXYL group,OXYH group and OXYM group was higher than IR group(P < 0. 05);The FINS level and HOMA - IR in IR group were higher than NC group (P < 0. 05). The HOMA - IR in OXYL group was lower than IR group(P < 0. 05). The FINS level,HOMA - IR in OXYH group and OXYM group were lower than IR group(P < 0. 05). The TC level in mice liver tissue of IR group was higher than NC group(P < 0. 05). The TC level in mice liver tissue of OXYL group,OXYM group and OXYH group was lower than IR group (P < 0. 05). The expressions of SREBP-2,HMGCR,LDLr mRNA and their protein in mice liver tissue of IR group were lower than NC group,and the expressions of SCAP,INSIG2 mRNA and their protein in mice liver tissue of IR group were higher than NC group(P < 0. 05). The expression of LDLr mRNA and its protein in mice liver tissue of OXYL group were higher than IR group(P < 0. 05). The expressions of SREBP-2,HMGCR,LDLr mRNA and their protein in mice liver tissue of OXYH group and OXYM group were higher than IR group,and the expressions of SCAP,INSIG2 mRNA and their protein in mice liver tissue of OXYH group and OXYM group were lower than IR group(P < 0. 05). Conclusion Oxymatrine could regulate the expression of genes related hepatic cholesterol and improve the blood glucose,lipid and insulin resistance in ApoE - / - mice induced by high fat diet.关键词
氧化苦参碱/膳食,高脂/ApoE -/- 小鼠/胰岛素抵抗/胆固醇Key words
Oxymatrine/Diet,high fat/ApoE - / - mice/Insulin resistance/Cholesterol分类
医药卫生引用本文复制引用
王杏,王超,宋光耀,费雯婕,刘小娜,张哲,马欢..氧化苦参碱对高脂饮食诱导胰岛素抵抗载脂蛋白 E基因敲除小鼠肝脏胆固醇代谢调控基因的影响[J].中国全科医学,2016,19(33):4067-4072,6.基金项目
国家自然科学基金资助项目（81370900）；河北省重大医学科研项目 （）
