中国全科医学2016，Vol.19Issue(33)：4087-4091,5.DOI:10.3969/j.issn.1007-9572.2016.33.013
金匮肾气丸防治创伤后应激障碍综合征孕鼠胎损的基因表达谱机制研究
Applying Jinkui Shengqi Pills in Pregnant Rats with PTSD to Treat Fetal Rats Injury:Gene Expression Profile and Mechanism
摘要
Abstract
Background The prenatal stress can cause fetal rats injury,it is closely related to congenital impairment- induced kidney qi damage. Jinkui Shengqi Pills can improve the kidney qi damage of pregnant rats with post traumatic stress disorder(PTSD),and can improve the pregnant rats′ PTSD - induced abnormal growth of offspring rats. Objective Jinkui Shengqi Pills were applied in pregnant rats with PTSD to treat fetal rats injury,the gene expression profile and mechanism were explored. Methods The study was implemented between July 2014 and March 2015,36 initial pregnant SD rats were divided into 3 groups by random number table method:control group(n = 12),PTSD group(n = 12),and Jinkui Shengqi Pill group (n = 12). The PTSD model rats were established during the second trimester(the 7th - 13th day after gestation)in PTSD group and Jinkui Shengqi Pill group. The rats in control group and PTSD group were fed with conventional food under normal experiment condition. The rats in Jinkui Shengqi Pill group were fed with food consisting of Jinkui Shengqi Pill until delivery. Gene expression of 30 days old rats was detected by gene expression profile chips. Results According to the gene expression profile analysis of 30 days old rats in PTSD group and blank group,there were 112 differentially expressed genes,including 70 highly expressed genes and 42 low expressed genes,among which 53 genes returned to normal by using Jinkui Shengqi Pills,these 53 genes were mainly involved in endocytosis pathway and MAPK signaling pathway. Conclusion Applying Jinkui Shengqi Pills in pregnant rats with PTSD can treat fetal rats injury,the underlying molecular biology mechanism is related to the differential expression of 53 genes, these 53 genes were mainly involved in endocytosis pathway and MAPK signaling pathway.关键词
金匮肾气丸/创伤后应激障碍综合征/基因表达谱/MAPK 信号通路/细胞内吞Key words
Jinkui Shengqi pills/Post traumatic stress disorder/Gene expression profiling/MAPK signaling pathway/Endocytosis pathway分类
医药卫生引用本文复制引用
王红艳,张辉,彭思涵,曹俊,谢汶倚,张先庚..金匮肾气丸防治创伤后应激障碍综合征孕鼠胎损的基因表达谱机制研究[J].中国全科医学,2016,19(33):4087-4091,5.基金项目
国家自然科学基金资助项目（81373710）；成都中医药大学科技发展基金项目 （）
