中国水产科学2016，Vol.23Issue(6)：1247-1254,8.DOI:10.3724/SP.J.1118.2016.16093
皱纹盘鲍眼部组织的显微及亚显微结构观察
Light and electron microscopic study of the eye ofHaliotis discus hannai Ino
摘要
Abstract
Light is a key environmental factor that influences the growth, culture and survival of aquatic organisms. In the present study, the eye tissues ofHaliotis discus hannaiIno were observed under the light microscope, scan-ning electron microscope and transmission electron microscope to provide an enhanced histological and cytologi-cal basis for analysis of the physiological response mechanism of abalone to light from the perspective of mo-lecular biology. Findings show that the eye tissues, from the outside to the inside, are: retinal pigment epithelium, outer nuclear layer, inner segment, inner nuclear layer, melanin granules sediment and optical fiber layer. Connec-tive tissue mainly exists between the epithelium and the outer nuclear layer, and a portion of the connective tissue nucleus is a darker color. The optical fiber layer mainly comprises the light red reticular nerve fibers which gradually become dense from the outside to the inside. The front ends of eye tissues are cylinder-shaped with an obvious circular depression, in which the reticular nerve fibers are mostly found. The surface of the eyestalk com-prises a large number of reticular fibers where the cavities of various sizes are densely distributed. The surfaces of eye tissues are covered with papillae and the top of every papilla has one or two clusters of ciliary rings. The ex-istence of desmosomes between adjacent cells can be clearly seen, and these can limit the expansion of cells and closely link them. Nucleiare found in various shapes and the double-layer nucleus is tightly enveloped by the cell membrane, which contains euchromatin and a few heterochromatin. Myofibrils are widely distributed and are composed of a large number of myofilaments closely arranged along the myofibrils; however, no obvious connec-tion is formed between them. Loose connective tissue, smooth muscle fiber and others are main components in the tissues. Collagenous and other types of fibers distributed between the connective tissues play a key role in main-taining the flexibility and toughness of cells. Mitochondria, Golgi apparatus and lysosomes are also widely dis-tributed in supporting cells. Amoebocytes are uniquely shaped and distributed between the loose connective tis-sues with pseudopodia and a large number of high electron density particles. Many melanin granules are densely distributed in the cytoplasm; these can prevent strong light from damaging the optical cells, and they also stabilize the internal environment of the retina. Findings demonstrate the significant role played by the optical tissues of the abalone in sensing and identifying external light environmental factors. They also provide a morphological basis for the optimization and regulation of light environmental factors in aquaculture production, as well as an in-depth discussion of the abalone’s physiological response mechanism to light.关键词
皱纹盘鲍/眼部组织/显微结构/亚显微结构Key words
Haliotis discus hannaiIno/eye/microstructure/ultrastructure分类
农业科技引用本文复制引用
高霄龙,张墨,李贤,宋昌斌,刘鹰..皱纹盘鲍眼部组织的显微及亚显微结构观察[J].中国水产科学,2016,23(6):1247-1254,8.基金项目
国家自然科学基金项目(31472312，41306152，31402283，31672673) （31472312，41306152，31402283，31672673）
国家贝类产业技术体系项目(CARS-48) （CARS-48）
国家“十二五”科技支撑计划项目(2014BAD08B09) （2014BAD08B09）
