中国水产科学2016，Vol.23Issue(6)：1300-1310,11.DOI:10.3724/SP.J.1118.2016.15455
共轭亚油酸对珍珠龙胆石斑鱼幼鱼生长、体组成及肝代谢相关酶活力的影响
Effects of dietary conjugated linoleic acid on growth, body composi-tion and metabolism-related hepatic enzyme activities in juvenile hy-brid grouper (Epinephelus fuscoguttatus♀ ×E. lanceolatu♂)
摘要
Abstract
We investigated how conjugated linoleic acids (CLA) can affect the growth, body composition and me-tabolism-related hepatic enzyme activities of juvenile hybrid grouper (♀Epinephelus fuscoguttatus×♂E. lanceolatu). Fishes with initial body weight of (63.57±0.56) g were examined after 8 weeks of feeding with isoni-trogenous and isolipidic diets in which 0 (CLA0), 0.8% (CLA0.8), 1.6% (CLA1.6), 2.4% (CLA2.4) and 3.2% (CLA3.2) of conjugated linoleic acid were supplemented. The results showed that: (1) there were no significant differences in weight gain ratio (WGR), survival rate (SR), specific growth rate (SGR), condition factor (CF), hepatosomatic index (HIS) and viscerosomatic index (VSI). The protein efficiency ratio (PER) increased as levels of dietary CLA rose, and was significantly higher in all experimental groups than in CLA0 groups (P<0.05). Feed intake (FI) was reduced with increasing dietary CLA; FI in group CLA0 was significantly higher than that in other groups (P<0.05). Feed conversion ratio (FCR) was decreased, and achieved in the group CLA2.4, which was sig-nificantly lower than CLA0 (P<0.05); (2) muscle fat content increased as amounts of dietary CLA rose, and the achieved in the group CLA0.8, which was significantly lower than that in group CLA0 (P<0.05). Liver fat content was not significant (P>0.05). The content of saturated fatty acid (SFA) and C18:0 in the muscle of group CLA1.6 was significantly higher than in group CLA0 (P<0.05), and monounsaturated fatty acid (MUFA) content in CLA3.2 achieved minimum as well as C18:1n-9, which was significantly lower than that of control group (P<0.05). Liver content of C16:0 and C18:0 in groups CLA2.4 and CLA3.2 were significantly higher than in the control group (P<0.05); however, C16:1n-7, C18:1n-9 and C18:1n-7 content were significantly lower than in group CLA0 (P<0.05). C18:3n-3and C18:2n-6content of muscle and liver in groups CLA2.4 and CLA3.2 were significantly lower than in group CLA0 (P<0.05). C18:2c9, t11 and C18:2t10, c12 content in muscle and liver were highly related with dietary CLA supplement, and in all experimental groups were significantly higher than in CLA0 groups (P<0.05); (3) dietary conjugated linoleic acid levels among glucose-6-phosphatedehydrogenase (G6PD) activity in the experimental groups were significantly higher than those in the control group (P<0.05); acyl-coenzyme aoxidase activity has the highest CLA3.2 activity, which was higher than in the other groups (P<0.05); general esterase and fatty acids yn-thetase activity in the experimental groups was significantly higher than in the control group (P<0.05).The results indicate that dietary CLA can significantly improve the utilization of aquafeed by juvenile hybrid grouper, impr-ove the composition of fatty acids in liver and muscle, and increase metabolism-related hepatic enzyme activities.关键词
共轭亚油酸/珍珠龙胆石斑鱼/生长性能/体组成/脂肪代谢Key words
conjugated linoleic acid/juvenile hybrid grouper (Epinephelus fuscoguttatus♀ ×E. lanceolatu♂)/growth performance/body composition/lipid metabolism分类
农业科技引用本文复制引用
王明辉,王际英,宋志东,李培玉,李宝山,李忠清,孙永智,张利民..共轭亚油酸对珍珠龙胆石斑鱼幼鱼生长、体组成及肝代谢相关酶活力的影响[J].中国水产科学,2016,23(6):1300-1310,11.基金项目
国家海洋公益性行业专项(201205025,201205028) （201205025,201205028）
国家海洋生物产业水生动物营养与饲料研发创新示范平台(201501005) （201501005）
国家自然科学基金青年科学基金(31201973) （31201973）
