中国社区医师2016，Vol.32Issue(32)：9-10,2.DOI:10.3969/j.issn.1007-614x.2016.32.2
支气管肺泡灌洗术在小鼠肺纤维化模型中的应用
Application of bronchoalveolar lavage in the model of pulmonary fibrosis in mice
邹叶强 1孟婕2
作者信息
- 1. 415000常德市第一人民医院 1
- 2. 410008中南大学湘雅医院 2
- 折叠
摘要
Abstract
Objective:To explore the feasibility of improving the recovery rate of bronchoalveolar lavage fluid(BALF)in mice with pulmonary fibrosis by improving the experimental method.Methods:Male BALB/C mice were selected.36 male mice from 6 to 8 week were divided into the 3 groups.After 28 days of pulmonary fibrosis,the bronchoalveolar lavage was performed 4 times,0.8 mL/times,and repeated 4 times.Macrophages and lymphocytes were the main inflammatory cells in the lavage fluid.Results:Through this method each mouse can be collected BALF about 2.6~2.8 mL/3.2 mL.There was no statistical difference between groups,and there was no significant difference between groups(P>0.05).The recovery rate was about 81.25%~87.5%,and the cell count could meet the experimental requirements.Conclusion:The recovery rate of alveolar lavage fluid obtained from this method can meet the need of medical scientific research,and it has the characteristics of strong operability and practicability.关键词
支气管肺泡灌洗/肺纤维化/小鼠Key words
Bronchoalveolar lavage/Pulmonary fibrosis/Mice引用本文复制引用
邹叶强,孟婕..支气管肺泡灌洗术在小鼠肺纤维化模型中的应用[J].中国社区医师,2016,32(32):9-10,2.
