中国药房2016，Vol.27Issue(32)：4504-4507,4.DOI:10.6039/j.issn.1001-0408.2016.32.13
表面增强拉曼光谱法检测人尿液、粪便中马来酸依那普利的浓度及其临床应用Δ
Concentration Determination of Enalapril Maleate in Human Urine and Feces by Surface-enhanced Raman Scattering Spectrometry and Its Clinical Application
摘要
Abstract
OBJECTIVE:To establish the method for concentration determination of enalapril maleate in human urine and fe-ces,and apply it in the clinic. METHODS:The urine and feces samples were collected and pre-processed,then tested by sur-face-enhanced Raman scattering spectrometry with ratio of sample to surface signal enhancer silver sol 1∶1(V/V). The assay was carried out with the following parameters:excitation power 400 mW,excitation wavelength 785 nm,resolution 4.5 cm-1. The char-acteristic shift of enalapril maleate were(800±2),(1 050±2),(1 330±2),(1 450±2),(1 630±2)cm-1. The quantitation shift was(1 050±2)cm-1. RESULTS:The linear range of enalapril maleate were 0.50-100.00 μg/ml in urine and 2.00-100.00 μg/g in feces(r=0.989 3,0.978 0,n=6);the limits of quantitation were 0.50 μg/ml and 2.00 μg/g,and the limits of detection were 0.10 μg/ml and 1.30 μg/g. RSDs of intra-day and inter-day were both lower than 5%. The method recoveries were 95.24%-99.38%and 86.73%-94.57%;the extraction recoveries were 84.29%-90.43%(RSD=0.84%)and 82.36%-87.70%(RSD=1.84%),respec-tively. The urinal concentration of enalapril maleate in 15 hypertensive patients ranged 15.31-33.46μg/ml,while the fecal concentra-tion ranged 5.08-41.79 μg/g. There was no statistical significance in average urinal concentration and fecal concentration between“effective”patients and“no response”patients. CONCLUSIONS:Surface enhanced Raman scttering spectrometry can be used for the concentration determination of enalapril maleate in human urine and feces. It is simple and rapid.关键词
马来酸依那普利/尿液/粪便/表面增强剂/表面增强拉曼光谱法Key words
Enalapril maleate/Urine/Feces/Surface signal enhancer/Surface-enhanced Raman scattering spectrometry分类
医药卫生引用本文复制引用
甘盛,赖青鸟,韩婷,吴超权..表面增强拉曼光谱法检测人尿液、粪便中马来酸依那普利的浓度及其临床应用Δ[J].中国药房,2016,27(32):4504-4507,4.基金项目
广西自然科学青年基金资助项目（No.2014GXNSF BA118059）；中国食品药品检定研究院中青年发展研究基金项目 （）
