中国中医药信息杂志2016，Vol.23Issue(12)：55-58,4.DOI:10.3969/j.issn.1005-5304.2016.12.014
糖尿病并发抑郁症大鼠海马神经元凋亡情况及潜在机制研究
Study on Hippocampal Neuronal Apoptosis and Its Potential Mechanisms in Diabetic Rats ;with Depression
摘要
Abstract
Objective To study hippocampal neuronal apoptosis in rats with diabetes mellitus and depression (DD);To explore its potential molecular mechanisms. Methods High-fat fed combined with intravenous injection of STZ was used to establish the models of diabetic rats, and then the models were divided into diabetes group and DD group after modeling successfully. Normal rats were randomly divided into control group and depression group. Chronic unforeseeable stress was continuously given to depression group and DD group. Open-field test and Morris water maze were used to evaluate the behavior activity of rats in each group; HE staining was used to detect pathological changes in hippocampus of rats;Western blot was used to disclose the protein expression of p-JNK, Bax, Bcl-2, Caspase8, and Caspase3. Results Open-field test results showed that the independent activity of rats decreased significantly in each model group, which decreased significantly in the DD group;Morris water maze results showed longer escape latency in model rats, which increased obviously in DD group;HE staining results suggested that the damage of hippocampus was the most severe in DD rats. Western blot results showed that compared with normal group, the expressions of apoptosis protein of p-JNK, Bax, Caspase8, and Caspase3 in each model group increased significantly and Bcl-2 expression decreased significantly, among which the difference in the DD group was the most obvious. Conclusion Hippocampal neuronal apoptosis caused by DD is based on diabetes, which mechanism may be related to abnormal expressions of p-JNK, Bax, Bcl-2, Caspase8, and Caspase3.关键词
糖尿病/抑郁症/糖尿病并发抑郁症/神经元凋亡Key words
diabetes mellitus/depression/diabetes mellitus with depression/neuronal apoptosis分类
医药卫生引用本文复制引用
赵洪庆,杜青,凌佳,杨琴,徐雅岚,王宇红..糖尿病并发抑郁症大鼠海马神经元凋亡情况及潜在机制研究[J].中国中医药信息杂志,2016,23(12):55-58,4.基金项目
国家自然科学基金 （）
