中国中医药信息杂志2016，Vol.23Issue(12)：96-98,3.DOI:10.3969/j.issn.1005-5304.2016.12.023
液相色谱-质谱法测定茜草中芦西丁含量
Content Determination of Lucidin in Rubiae Radix et Rhizoma by LC-MS
摘要
Abstract
Objective To develop a LC-MS method for the content determination of lucidin in Rubiae Radix et Rhizoma. Methods Agilent 1290 HPLC and G6460 Mass were used for quantification in MRM mode under ESI-. chromatography was performed on a Agilent Eclipse Plus C18 RRHD column (2.1 mm × 50 mm, 1.8 µm) at 30 ℃using acetonitrile-0.1% formic acid in water at the flow rate of 0.4 mL/min. The injection volume was 2 µL. Results Good linearity was obtained from concentration of lucidin among the range of 0.005 2–2.64 ng (r=0.999 9). Recoveries at different concentrations (80%, 100%, 120%) were between 94.80% and 100.94% (AVE=97.17%, RSD=6.16%). Good reproducibility and precision were also achieved. Conclusion The new developed LC-MS method is sensitive, accurate and with good repeatability, which is suitable for the content determination of lucidin in Rubiae Radix et Rhizoma. It can be used in the limit detection of lucidin in Rubiae Radix et Rhizoma.关键词
茜草/芦西丁/液相色谱-质谱联用Key words
Rubiae Radix et Rhizoma/lucidin/LC-MS分类
医药卫生引用本文复制引用
浦益琼,丁越,王冰,张若曦,张彤..液相色谱-质谱法测定茜草中芦西丁含量[J].中国中医药信息杂志,2016,23(12):96-98,3.基金项目
上海市科学技术委员会科研计划项目（11DZ1971500）；上海市卫生局青年科研项目 （）
