动物营养学报2016，Vol.28Issue(11)：3511-3519,9.DOI:10.3969/j.issn.1006-267x.2016.11.018
肉鸡肥胖基因 WDTC1组织表达特性及油脂类型和水平对 WDTC1基因表达的影响
Tissue Expression Characteristics of Obesity Gene WDTC1 of Broilers and Effects of Oil Type and Level on WDTC1 Gene Expression
摘要
Abstract
Broiler obesity gene WDTC1 ( WD and tetratricopeptide repeats 1) , a homologous gene of mouse adipose gene, has become a key gene in regulating energy metabolism, fat deposition and serum triglyceride level. This experiment was conducted to analyze the tissue expression profile of WDTC1 gene in broilers and the effects of different types and different levels of oil on WDTC1 gene expression of broilers in different tissues and at different days of age. Five commercial female broilers at 42 days of age were randomly selected to anal⁃ysis the tissue expression profile of WDTC1 gene in broilers. On this basis, two hundred and forty 1⁃day⁃old Cobb female broilers were randomly assigned to 8 groups ( fed basal diets added with 2.50% and 5.00% lin⁃seed oil, corn oil, sesame oil and lard oil, respectively) with 6 replicates per group and 5 broilers per repli⁃cate. One broiler of each replicate was selected to slaughter at age of 21 days and 42 days, respectively. The interactive effects of oil type, oil level and tissue of broilers and oil type, oil level and days of age of broilers on WDTC1 gene of broilers were designed to analysis by three factors factorial experiment design. The results showed as follows:1) WDTC1 gene was found to be widely expressed in various tissues of broilers, and the expression level was the highest in the ovary, then in liver, kidney, brain, lung, breast muscle, heart and small intestine, and the expression level was the lowest in pancreas. 2) The relative expression level of WDTC1 gene in different tissues of broilers at 42 days of age was significantly affected by the oil type ( P<0.01) , and the interactive effects of oil type and tissue of broilers ( P<0.01) and oil type and oil level ( P<0.05) also had significant effects on the relative expression level of WDTC1 gene. The relative expression level of WDTC1 gene in liver of broilers at 42 days of age in linseed oil group at 2.50% and 5.00% oil levels was significantly higher than that in abdominal fat ( P<0.05) , and the relative expression level of WDTC1 gene in liver in lin⁃seed oil group was significantly higher than that in sesame oil group ( P<0.05) . The relative expression level of WDTC1 gene in abdominal fat of broilers in sesame oil group at 2.50% oil level was significantly higher than that in linseed oil group ( P<0.05) . Oil type and level had no significant effect on the relative expression level of WDTC1 gene in breast muscle of broilers at 42 days of age ( P>0.05) . 3) The relative expression level of WDTC1 gene in liver of broilers at different days of age was significantly affected by the oil type ( P<0.01) , and the interactive effect of oil type and oil level also had significant effect on the relative expression level of WDTC1 gene ( P<0.05) . The relative expression level of WDTC1 gene in liver of broilers at 21 days of age in lard oil group at 2.50% and 5.00% oil levels was significantly higher than that at 42 days of age (P<0.05). The relative expression level of WDTC1 gene in liver in linseed oil group at 2.50% oil level was significantly higher than that in lard oil ( P<0.05) , and the relative expression level of WDTC1 gene in liver of broilers at 42 days of age in linseed oil and corn oil groups at 5.00% oil level was significantly higher than that in linseed oil group ( P<0.05) . In conclusion, WDTC1 gene expression of broilers has the temporal and spatial specifici⁃ties, and liver is the predominantly expression tissue. WDTC1 expression level is affected by oil type, tissue of broilers, days of age of broilers and the interaction effects of oil type and oil level, oil type and tissue of broil⁃ers.关键词
肉鸡/油脂/WDTC1/基因表达Key words
broilers/oil/WDTC1/gene expression分类
农业科技引用本文复制引用
王婷,陶亚飞,陈青,陈文,黄艳群..肉鸡肥胖基因 WDTC1组织表达特性及油脂类型和水平对 WDTC1基因表达的影响[J].动物营养学报,2016,28(11):3511-3519,9.基金项目
国家自然科学基金 （）
