动物营养学报2016，Vol.28Issue(11)：3520-3527,8.DOI:10.3969/j.issn.1006-267x.2016.11.019
皮质醇对斜带石斑鱼原代培养肝细胞糖代谢的影响
Effects of Cortisol on Glycometabolism in Primary Cultured Hepatocytes form Epinephelus coioides
摘要
Abstract
This experiment was conducted to study the effects of cortisol on glycometabolism in primary cul⁃tured hepatocytes form Epinephelus coioides. Freshly hepatocytes were isolated from Epinephelus coioides, and cultured at different cortisol concentrations [0 (control), 100 and 1 000 nmol/L] and different incubation time ( 24 and 36 h) . Medium glucose content ( hepatocyte glucose production) , hepatocyte glycogen and pyru⁃vate contents, and the activities of glycometabolism related enzymes including phosphoenolpyruvate carboxyki⁃nase ( PECK ) , glucose⁃6⁃phosphatase ( G6Pase ) , glycogen synthase ( GSase ) , malate dehydrogenase ( MDH) , isocitrate dehydrogenase ( ICD) and pyruvate kinase ( PK) were assessed at 24 and 36 h of incuba⁃tion, respectively. The results showed as follows: compared with the control group, the hepatocyte glucose production in 100 and 1 000 nmol/L cortisol groups was significantly increased ( P<0.05) , but the hepatocyte pyruvate content was significantly decreased ( P<0.05) , both at 24 and 36 h of incubation. The time of incu⁃bate to cortisol showed no significant effect on hepatocyte glucose production and pyruvate content ( P>0.05) . At 24 h of incubation, cortisol concentration showed no significant effect on hepatocyte glycogen content ( P>0.05) , however, at 36 h of incubation, hepatocyte glycogen content was significantly decreased with cortisol concentration increasing ( P<0.05) . With the cortisol incubation time extending, hepatocyte glycogen content was significantly decreased ( P<0.05) . At 24 and 36 h of incubation, 100 and 1 000 nmol/L cortisol signifi⁃cantly increased the activities of hepatocyte PEPCK and G6Pase ( P<0.05) , and significantly decreased the ac⁃tivities of hepatocyte MDH and ICD ( P<0.05) , however, there was no significant difference in the activity of hepatocyte GSase ( P>0.05) . At 24 h of incubation, cortisol concentration showed no significant effect on the activity of hepatocyte PK ( P>0.05) , but at 36 h of incubation, 100 and 1 000 nmol/L cortisol significantly increased the activity of hepatocyte PK ( P<0.05) . With the cortisol incubation time extending, the activity of hepatocyte G6Pase was significantly decreased (P<0.05), the activity of hepatocyte PK was significantly in⁃creased (P<0.05), however, there were no significant differences in the activities of hepatocyte MDH and ICD ( P>0.05) . The present study demonstrates that cortisol can enhance the gluconeogenesis, and has no reg⁃ulating effect on synthesis of hepatic glycogen, but inhibits the catabolism of glucose of the primary cultured hepatocytes from Epinephelus coioides.关键词
斜带石斑鱼/原代培养/皮质醇/糖代谢/酶活性Key words
Epinephelus coioides/primary cultured/cortisol/glycometabolism/enzyme activities分类
农业科技引用本文复制引用
宋凯,骆源,张春晓,王玲,游文煌,陈晓卉,阳达..皮质醇对斜带石斑鱼原代培养肝细胞糖代谢的影响[J].动物营养学报,2016,28(11):3520-3527,8.基金项目
国家自然科学基金青年科学基金项目（31302198）；国家公益性行业（农业）专项（201303053）；集美大学大学生创新性试验计划项目 （）
