动物营养学报2016，Vol.28Issue(11)：3542-3548,7.DOI:10.3969/j.issn.1006-267x.2016.11.022
猪源益生芽孢杆菌的分离鉴定与生物学特性分析
Isolation, Identification and Biological Characterization of Bacillus spp from Swine
摘要
Abstract
The aim of this project was to isolate Bacillus spp with good performance from the normal intestinal flora of healthy swine, and to improve the feed utilization rate, reduce the infection of pig digestive tract and reduce the use of antibiotics. The feces specimen were collected from local back⁃yard cows which never feed with manufactured feeds, and the Bacillus spp with acid and bile salt tolerance was isolated from fresh feces. The isolates were then tested for the production potency of amylase, cellulose and protease on specific agar plates. The strains were identified by biochemical assays and 16s rDNA sequence alignment analysis. The re⁃sults showed that 7 Bacillus strains were isolated which could secrete amylase, cellulose and proteinase. The survival ratios of Bacillus strains in pH 3.0 for 2 hours were 30% to 90%, and in 0.5% bile salt for 4 hours were 40% to 100%. The strains were identified as Bacillus stearothermophiluc ( 1 strain) , Bacillus lichenifor⁃mis ( 1 strain) and Bacillus subtilis ( 5 strains) by biochemical identification and molecular identification, re⁃spectively. In conclusion, it selects 7 strains identified Bacillus spp, which have good tolerance in low pH and high bile salt conditions, and the strains can secrete amylase, cellulose and proteinase.关键词
益生菌/芽孢杆菌/生化鉴定/16S rDNA/产酶能力Key words
probiotics/Bacillus spp/biochemical identification/16S rDNA/enzyme production capacity分类
农业科技引用本文复制引用
蒋佳璇,王淑京,任志鸿,杜华茂..猪源益生芽孢杆菌的分离鉴定与生物学特性分析[J].动物营养学报,2016,28(11):3542-3548,7.基金项目
传染病预防控制国家重点实验室留学归国人员启动经费 （）
