动物营养学报2016，Vol.28Issue(11)：3590-3601,12.DOI:10.3969/j.issn.1006-267x.2016.11.028
基于康奈尔净碳水化合物与蛋白质体系的瘤胃非降解蛋白质小肠可吸收氨基酸流量的简化评定技术
Simplification of Assessment Technology of Intestinal Absorbed Amino Acids Fluxes of Rumen Undegradable Protein Based on Cornell Net Carbohydrate and Protein System
摘要
Abstract
This study was conducted to simplify the assessment technology of intestinal absorbed amino acids fluxes of rumen undegradable protein ( RUP) based on Cornell net carbohydrate and protein system ( CNCPS) . Intestinal absorbed amino acids were consisted of microbial protein and RUP. Crude protein ( CP) was divided into A, B1, B2, B3 and C in CNCPS based on solubility, only B fractions could pass rumen and be digested in intestine. To estimate the contribution of intestinal absorbed amino acids from RUP, CNCPS model needed the degradability of B fractions, and intestinal digestibility of B fractions used different constants. This study collected 18 kinds of feedstuffs including 12 kinds of concentrate and 6 kinds of roughage. The data for measur⁃ing RUP intestinal absorbed amino acids fluxes in CNCPS was determined, and it was also determined that ru⁃minal degradability of CP and intestinal digestibility of RUP at different time points. Based on the above data, simplification methods were concluded. The results showed as follows: 1 ) CP ruminal degradability at 8 h ( X, %) and effective degradability ( Y, %) got the highest correlation with linear correlation both in concen⁃trates and roughages. The equations for concentrates and roughages were Y=12.652+0.828X, r=0.990, P<0.000 1 and Y=10.967+0.886X, r=0.980, P=0.000 6, respectively. 2) CP intestinal digestibility of resi⁃dues retained in rumen for 2 h ( X, %) got the highest correlation with RUP intestinal effective digestibility (Y, %) for concentrates, and the equation was Y=0.026+0.879X, r=0.970, P<0.000 1; CP intestinal di⁃gestibility of residues retained in rumen for 8 h ( X,%) got the highest correlation with RUP intestinal effective digestibility ( Y,%) for roughages, and the equation was Y=-0.002+0.960X, r=0.995, P<0.000 1. 3) Af⁃ter reliability assessment, the highest correlations were found in intestinal absorbed amino acids predicated by simplified CNCPS models using ruminal degradability and CP intestinal digestibility of residues retained in ru⁃men at 8 h ( X,‰) and CNCPS ( Y,‰) , and the equations for concentrates and roughages were Y=-0.056+1.409X, r=0.999, P<0.000 1 and Y=0.003+2.120X, r=0.999, P<0.000 1, respectively. The root⁃mean⁃square phase errors were 0. 245 and 0. 005, respectively, and the coefficients of variation were 7. 08% and 4.49%, respectively. In conclusion, simplified predation models for RUP intestinal absorbed amino acids fluxes are set based on CNCPS, and the simplified modes for concentrates and roughages are Y=-0. 056+1. 409 × [AA×(100-D8)×CP×ID8], r=0.999, P<0.000 1 and Y=0.002+2.120×[AA×(100-D8)×CP×ID8], r=0.999, P<0.000 1, respectively [ Y is RUP intestinal absorbed amino acid flux (‰) , D8 is CP ruminal de⁃gradability at 8 h (%) , ID8 is RUP intestinal digestibility at 8 h (%) , AA is amino acid content in insoluble protein (%)].关键词
康奈尔净碳水化合物与蛋白质体系/瘤胃降解率/消化率/氨基酸流量Key words
CNCPS/rumen degradation/digestibility/amino acids fluxes分类
农业科技引用本文复制引用
胡志勇,陶鲲,李延涛,刘晓莲,林雪彦,王中华..基于康奈尔净碳水化合物与蛋白质体系的瘤胃非降解蛋白质小肠可吸收氨基酸流量的简化评定技术[J].动物营养学报,2016,28(11):3590-3601,12.基金项目
国家自然科学基金（31572427，31372340）；国家现代农业（奶业）产业技术体系（CARS-37）；山东省牛产业技术体系 （）
