动物营养学报2016，Vol.28Issue(11)：3618-3625,8.DOI:10.3969/j.issn.1006-267x.2016.11.031
谷氨酸对脂多糖刺激断奶仔猪肠道能量代谢的影响
Effects of Glutamate on Intestinal Energy Metabolism in the Lipopolysaccharide-Challenged Weaned Piglets
摘要
Abstract
This study was aimed to investigate the effects of glutamate ( Glu) on intestinal energy metabolism in the lipopolysaccharide ( LPS)⁃challenged weaned piglets. Twenty⁃four weaned pigs were assigned to four groups as control group, LPS group, LPS+1.0% Glu group and LPS+2.0% Glu group with 6 replicates each and 1 pig in per replicate. On the 28th day of the trial, the piglets in the experimental groups were injected with 100 μg/kg BW LPS, and the piglets in the control group were injected with the same amount of 0.9% NaCl solution. At 4 h post⁃injection, pigs were slaughtered and intestinal samples were collected for further analysis. The results showed as follows: 1 ) LPS challenge significantly decreased ATP and total adenine nucleotide (TAN) contents and energy charge (P<0.05), but significantly increased the ratio of AMP to ATP (P<0.05) in jejunum compared with the control group; LPS+2.0% Glu group significantly increased the contents of ATP, ADP and TAN ( P<0. 05) in jejunum compared with LPS group. 2) Compared with the control group, LPS challenge had a tendency to decrease the alpha⁃oxoglutarate dehydrogenase complex activity ( P=0.092) in jejunum and extremely significantly decreased the activities of citrate synthase and alpha⁃oxoglutarate dehydrogenase complex ( P<0.01) in ileum; compared with LPS group, Glu had no significant effects on the activities of tricarboxylic acid cycle key enzymes ( P>0.05) in jejunum and ileum, except for the citrate syn⁃thase activity in ileum was significantly reduced in LPS+1.0% Glu group (P<0.05). 3) LPS challenge ex⁃tremely significantly decreased the mRNA expressions of peroxisome proliferator⁃activated receptor gamma co⁃activator⁃1α ( PGC1α) in jejunum and silent information regulator 1 ( Sirt1) and PGC1α in ileum ( P<0.01) compared with the control group;compared with LPS group, LPS+2.0% Glu group had a tendency to increase the mRNA expressions of PGC1α in jejunum ( P=0.067) and Sirt1 in ileum ( P=0.053) , while LPS+1.0%Glu group had a tendency to increase the Sirt1 mRNA expression in ileum ( P=0.070) . These results indicate that dietary supplementation of Glu can improve energy loss status in LPS injured intestine.关键词
谷氨酸/脂多糖/断奶仔猪/肠道/能量代谢Key words
glutamate/lipopolysaccharide/weaned piglets/intestine/energy metabolism分类
农业科技引用本文复制引用
秦琴,王秀英,吴欢听,朱惠玲,刘玉兰..谷氨酸对脂多糖刺激断奶仔猪肠道能量代谢的影响[J].动物营养学报,2016,28(11):3618-3625,8.基金项目
湖北省教育厅优秀中青年科技创新团队项目 （）
