动物营养学报2016，Vol.28Issue(11)：3634-3641,8.DOI:10.3969/j.issn.1006-267x.2016.11.033
饲粮粗蛋白质水平对猪胃肠道钙敏感受体基因表达、胃肠激素分泌及胃功能性酶活性的影响
Effects of Dietary Crude Protein Level on Calcium Sensing Receptor Gene Expression of Gastrointestinal Tract, Gastrointestinal Hormone Secretion and the Activities of Gastric Function Enzymes of Pigs
摘要
Abstract
The experiment was conducted to investigate the effects of different dietary crude protein ( CP) lev⁃els on calcium sensing receptor ( CaSR) gene expression of gastrointestinal tract, gastrointestinal hormone se⁃cretion and the activities of gastric function enzymes ( pepsin and H+⁃K+⁃ATPase) of pigs, and to explore the relationships between CaSR gene expression level of small intestine and the concentration of gastrointestinal hor⁃ mone in serum, CaSR gene expression level of stomach and the activities of H+⁃K+⁃ATPase and pepsin, re⁃spectively. Eighteen 35⁃day⁃old“Duroc×Landrace×Large White” crossed weaned pigs with initial body weight of (9.57±0.64) kg were randomly assigned into 3 group with 6 replicates per group and 1 pig per replicate, including NP group ( NRC standard CP level group ) , MP group ( decreased 3% CP level compared to the standard CP level group) and LP group ( decreased 6% CP level compared to the standard CP level group) . According to the nutrient needs of pigs in different growth periods, pigs were fed diets contained 20% ( NP group), 17% (MP group) and 14% (LP group) CP levels in weaned pigs period (35 to 80 days of age), re⁃spectively; diets contained 18% ( NP group) , 15% ( MP group) and 12% ( LP group) CP levels in growing pigs period ( 81 to 110 days of age) , respectively; diets contained 16% ( NP group) , 13% ( MP group) and 10% ( LP group) CP levels in finishing pigs period ( 111 to 160 days of age) , respectively. The levels of diet⁃ary lysine ( Lys) , methionine ( Met) , threonine ( Thr) and tryptophan ( Trp) were balanced. The experimen⁃tal period lasted for 125 days. Precaval vein blood were collected by the end of the experiment, then all pigs were slaughtered, and gastric chyme, stomach, duodenum, jejunum and ileum tissues and mucosa were col⁃lected, to determination of the concentration of gastrointestinal hormones in serum, the activities of pepsin of gastric chyme and H+⁃K+⁃ATPase of gastric mucosa and CaSR gene expression level of each tissues of the gas⁃trointestinal tract. The results showed as follows: 1 ) CaSR gene expression level of stomach of pigs in LP group was significantly higher than that in NP and MP groups ( P<0.05) , and CaSR gene expression level of duodenum and jejunum in MP and LP groups was significantly lower than that in NP group ( P<0.05) , but no significant difference was found in CaSR gene expression level of ileum among all groups ( P>0.05) . 2) Com⁃pared with NP group, the concentrations of peptide tyrosine tyrosine ( PYY) and glucose insulinotropic peptide (GIP) in serum of pigs in MP and LP groups were significantly decreased (P<0.05), the concentration of cholecystokinin ( CCK) in serum in MP group was significantly increased ( P<0.05) , and the activities of pep⁃sin of gastric chyme and H+⁃K+⁃ATPase of gastric mucosa in LP group were significantly increased ( P<0.05) . 3) CaSR gene expression level in stomach had a significantly positive correlations with the activities of pepsin of gastric chyme and H+⁃K+⁃ATPase of gastric mucosa (P<0.05), CaSR gene expression level in duodenum had a significantly positive correlation with the concentration of GIP in serum (P<0.05), and had a positive correlation tendency with the concentration of PYY in serum (0.05≤P<0.10), CaSR gene expression level in jejunum had a significantly positive correlations with the concentrations of GIP and PYY in serum ( P<0.05) , and CaSR gene expression level in ileum had a positive correlation tendency with the concentration of PYY in serum (0.05≤P<0.10). In conclusion, reduction of dietary CP level affects CaSR gene expression of gastro⁃intestinal tract, the activities of gastric function enzymes and gastrointestinal hormone secretion.关键词
猪/粗蛋白质水平/钙敏感受体/胃肠激素/H+-K+-ATP酶/胃蛋白酶Key words
pigs/crude protein level/calcium sensing receptor/gastrointestinal hormone/H+-K+-ATPase/pepsin分类
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县怡涵,赵秀英,丁立人,孟祥龙,王超,朱伟云,杭苏琴..饲粮粗蛋白质水平对猪胃肠道钙敏感受体基因表达、胃肠激素分泌及胃功能性酶活性的影响[J].动物营养学报,2016,28(11):3634-3641,8.基金项目
国家973项目（2013CB127301）资助 （）
