动物营养学报2016，Vol.28Issue(11)：3711-3720,10.DOI:10.3969/j.issn.1006-267x.2016.11.042
混菌固态发酵白酒糟开发为蛋白质饲料的条件优化及营养价值评定
Distillers Grains：Optimization of Mixed Bacterial Solid-State Fermentation Conditions to Produce Protein Feed and Nutrient Value Analysis
摘要
Abstract
This experiment was conducted to optimize mixed bacterial ( Geotrichum candidum, Aspergillus oryzae, Trichoderma viride and Bacillus subtils) solid⁃state fermentation conditions to produce protein feed, and analyzed the nutrient value. Geotrichum candidum, Aspergillus oryzae, Trichoderma viride and Bacillus subtils (1∶1∶1∶1) was added to culture medium at 10%. L16(54)orthogonal testing design was used, and 5 fer⁃mentation conditions with 4 variables were set ( 16 groups of fermentation conditions in all) . The conditions were base materials, urea, potassium di⁃hydrogen phosphate, pH and moisture. The mixtures prepared accord⁃ing to the conditions were cultured under (30±2) ℃ for 72 h. Range analyses for true protein and crude fiber contents before and after fermentation were carried out to determine the optimal conditions, and comparisons of nutrient levels and amino acid composition of distillers grains between before and after fermentation under opti⁃mal condition were done. The results showed as follows:1) the optimal combination was that base materials of 80% distillers grains, 10% wheat bran, 5% corn flour and 5% rapeseed meal supplemented with 1.5% adding urea and 0.7% potassium di⁃hydrogen phosphate with pH 5 and 50% moisture. 2) True protein content of dis⁃tillers grains after fermentation improved by 57.85% compared with that before fermentation ( P<0.01); the contents of crude fiber, acid detergent fiber, neutral detergent fiber and ether extract were reduced by 42.39%, 31.95%, 27.73% and 21.48% ( P<0.01) , respectively; and the contents of calcium and phosphorus were in⁃creased by 16.67% and 68.18% ( P<0.01) , respectively;total amino acids content was increased by 24.47%, and the contents of lysine, methionine, threonine, valine, leucine, isoleucine and proline were increased by 109.68%, 38.09%, 39.39%, 71.43%, 28.93%, 10.87% and 3.70%, respectively. In conclusion, the opti⁃mal conditions for of distillers grains solid⁃state fermentation using Geotrichum candidum, Aspergillus oryzae, Trichoderma viride and Bacillus subtils are as follows: base materials of 80% distillers grains, 10% wheat bran, 5% corn flour and 5% rapeseed meal supplemented with 1. 5% urea and 0. 7% potassium di⁃hydrogen phosphate with pH 5 and 50% moisture. True protein content of fermented distillers grains is 24.34.关键词
白酒糟/混菌/固态发酵/营养价值/优化Key words
distillers grains/mixed bacteria/solid-state fermentation/nutrient value/optimum分类
农业科技引用本文复制引用
张玉诚,薛白,达勒措,李秋瑾,何宇..混菌固态发酵白酒糟开发为蛋白质饲料的条件优化及营养价值评定[J].动物营养学报,2016,28(11):3711-3720,10.基金项目
国家现代农业产业技术体系四川区域创新团队 （）
