法医学杂志2016，Vol.32Issue(5)：342-345,4.DOI:10.3969/j.issn.1004-5619.2016.05.006
多重置换扩增在含抑制物检材中的应用
Application of Multiple Displacement Amplification in Samples with Inhibitors
丁东雪 1丁梅1
作者信息
- 1. 中国医科大学法医学院法医血清教研室，辽宁 沈阳 110001
- 折叠
摘要
Abstract
ObjectiveTo explore the ability of inhibition resistibility of multiple displacement amplification (MDA)in samples with inhibitors. To explain the application and value of MDA in forensic medicine by comparing with using magnetic beads methods(MBM)to purify sample.MethodsDifferent concentra-tions of hemoglobin and humid acid(HA)mixed with DNA samples and then divided the samples into MDA group, MBM group and control group.D3S1358locus was amplified and detected by polyacry-lamide gel electrophoresis detection system and AmpFℓSTR® IdentifilerTM Plus Kit-capillary electrophore-sis detection system.ResultsWhen hemoglobin concentrations exceed 1 ng/μL or HA concentrations ex-ceed 0.1 ng/μL, amplification products could not be obtained by single-locus system in control group. When hemoglobin concentration exceeds 100 ng/μL or HA concentrations exceed 1 ng/μL, the samples could not be amplified by MBM. Inhibitors in different concentrations were amplified successfully in MDA group without any influence from inhibitors.ConclusionMDA has the capability to remove the inhibi-tion of hemoglobin and HA, which is better than MBM and has a certain value in forensic practices.关键词
法医遗传学/核酸扩增技术/多重置换扩增/抑制物/血红素/腐殖酸Key words
forensic genetics/nucleic acid amplification techniques/multiple displacement amplification/inhibitors/hemoglobin/humic acid分类
社会科学引用本文复制引用
丁东雪,丁梅..多重置换扩增在含抑制物检材中的应用[J].法医学杂志,2016,32(5):342-345,4.
