法医学杂志2016，Vol.32Issue(5)：356-362,7.DOI:10.3969/j.issn.1004-5619.2016.05.010
湖南汉族人群21个STR基因座的遗传多态性（英文）
Genetic Polymorphisms of 21 STR Loci in Hunan Province-based Han Population
摘要
Abstract
ObjectiveTo investigate the genetic polymorphisms of 21 short tandem repeat(STR)loci (D3S1358, D13S317, D7S820, D16S539, Penta E, D2S441, TPOX, TH01, D2S1338, CSF1PO, Penta D, D10S1248, D19S433, vWA, D21S11, D18S51, D6S1043, D8S1179, D5S818, D12S391 and FGA). Methods A total of 560 blood samples were collected from unrelated healthy individuals of Han population in Hunan Province. Chelex-100 extraction method was applied to the extraction of genomic DNA, and an AGCU EX22 Kit and 9700 STR amplification was used in amplification reactions. The products were separated and analyzed on 310 Genetic Analyzer.ResultsA total of 248 alleles were observed, the al-lelic frequencies ranging from 0.001 to 0.518. Observation of genotype distributions for each locus showed no deviations from Hardy-Weinberg equilibrium exceptPentaE(P=0.023). The combined pow-er of discrimination, combined power of exclusion, and combined matching probability of the 21 STR loci were approximately 0.999 999 999 999 999 999 999 999 8, 0.999 999 998, and 1.36×10-25, respectively. ConclusionThe 21 STR loci show high polymorphisms in the Han population, which can provide valu-able data and a theoretical basis for forensic individual identification and paternity testing.关键词
法医遗传学/多态现象,遗传/短串联重复序列/湖南/汉族Key words
forensic genetics/polymorphism, genetic/short tandem repeat sequences/Hunan/Han nationality分类
社会科学引用本文复制引用
邹鹰,兰玲梅,郭娟娟,李情鹏,左道宏,刘金山,郭亚东,闫杰,扎拉嘎白乙拉,蔡继峰..湖南汉族人群21个STR基因座的遗传多态性（英文）[J].法医学杂志,2016,32(5):356-362,7.基金项目
This study was supported by the Natural Science Foundation for the Youth（No.81302621）and the Fundamental Research Funds for the Central Uni-versities of Central South University （）
