中国妇幼健康研究2016，Vol.27Issue(10)：1207-1209,3.DOI:10.3969/j.issn.1673-5293.2016.10.014
乳腺癌局部微环境中免疫相关细胞因子mRNA表达状态及临床病理意义
Expression status of immune related cytokines mRNA in local microenvironment of breast cancer and its clinicopathologic significance
崔刚 1张娜娜 1阎艳 1邓宝国 1胡业佳 1王鸿雁 2王一理1
作者信息
- 1. 西安交通大学基础医学院癌症研究所,陕西西安,710061
- 2. 西安交通大学第一附属医院病理科,陕西西安,710061
- 折叠
摘要
Abstract
Objective To investigate the expression status of immune related cytokines IFN-γ, IL-10 and TGF-βmRNA in breast cancerous microenvironment and make comparison with clinicopathologic features to explore its significance.Methods Forty-eight samples of breast cancer with excised axillary lymph nodes were included.The samples were divided into two groups according to lymph node metastatic status.Digoxin-labeled oligonucleotide probes and in situ hybridization technique were used to detect the expressions of IFN-γ, IL-10 and TGF-βmRNA in breast cancer cells and the tumor infiltrating mononuclear cells.Results The expressions of TGF-βand IL-10 mRNA in breast cancerous cells were significantly higher in node metastatic group compared to those of non-metastatic group ( TGF-β[%/accumulative index]:46.25 ±12.88/0.49 ±0.27 vs 19.33 ±7.69/0.28 ±0.18; IL-10[%/accumulative index]: 39.08 ±17.49/0.42 ±0.19 vs 22.58 ±7.87/0.33 ±0.17, t=4.330, P<0.01;t=2.962, P<0.05).The difference in expression of TGF-βmRNA in breast cancer cells was more significant.IFN-γmRNA positive cancer cell was not detected.In tumor infiltrating mononuclear cells, the IFN-γmRNA positive cells in node metastatic group were significantly less than TGF-βand IL-10 mRNA positive cell (TGF-β:35.58 ± 13.62/0.40 ±0.25, IL-10:40.83 ±15.49/0.47 ±0.22, IFN-γ:18.17 ±8.41/0.18 ±0.08, t=3.005, P<0.05;t=4.390, P<0.01;t=3.563, P<0.01).Conclusion Treg and Th2 related cytokines are predominated in local microenvironment of breast cancer and the microenvironments of breast cancer demonstrate an immunoinhibitory status in terms of anticancer immune response.关键词
乳腺癌/细胞因子/原位杂交/信使核糖核酸(mRNA)Key words
breast cancer/cytokine/in situ hybridization/mRNA分类
医药卫生引用本文复制引用
崔刚,张娜娜,阎艳,邓宝国,胡业佳,王鸿雁,王一理..乳腺癌局部微环境中免疫相关细胞因子mRNA表达状态及临床病理意义[J].中国妇幼健康研究,2016,27(10):1207-1209,3.
