临床与病理杂志2016，Vol.36Issue(10)：1562-1568,7.DOI:10.3978/j.issn.2095-6959.2016.10.019
沉默RORα通过Wnt信号通路促进人胃癌细胞增殖与迁移侵袭
Silencing RORαpromote proliferation, migration and invasion through Wnt signaling pathway in MGC803 cells
摘要
Abstract
Objective:To investigate Silencing RORαthrough Wnt signaling pathway promote proliferation, migration and invasion in human gastric MGC803 cells underlaying construction of MGC803 cells of silencing RORα. Methods:Colony formation and lfow cytometry were used for cell proliferation and cell cycle. Wound healing and transwell assays were performed to examine cell migration and invasion activities. hTe expression of RORα, Wnt1, MMP-9 and TIMP3 mRNA and protein were detected by Western blot and RT-PCR. Results:hTe colony forming showed that the colony forming efficiency in miR-RORα group was 128.1% higher significantly than in control and vector/ MGC803 (P<0.05). The flow cytometry revealed that percentage in S cycle was 39.9% in miR-RORα markedly increase than 26.1% in control and 27.4% in vector/MGC803 (P<0.05). The migration experiment disclosed that migration distance in miR-RORα was (1.76±0.19) mm notably increase than (1.32±0.14) mm in control and (1.29±0.17) mm in vector/MGC803 (P<0.05). The invasion essay exhibited that the cells of passed through the Matrigel were (172±27) in miR-RORα increased than (147±17) in control and (149±14) in vector/MGC803, respectively (P<0.05). RT-PCR and Western blot indicated that silencing RORα up-regulated expression of wnt1 and MMP-9 and down-regulated expression of RORα and TIMP3, compared with control and vector/MGC803, respectively (P<0.05). Conclusion: Silencing RORα can promote proliferation, migration and invasion through Wnt signaling pathway in human gastric MGC803 cells.关键词
RORα沉默/人胃癌MGC803细胞/增殖/迁移/侵袭/Wnt通路Key words
silencing RORα/human gastric cancer MGC803 cells/proliferation/migration/invasion/Wnt pathway引用本文复制引用
刘芳,赵晓红,夏红,苏坚,曾希,苏波,凌晖,苏琦..沉默RORα通过Wnt信号通路促进人胃癌细胞增殖与迁移侵袭[J].临床与病理杂志,2016,36(10):1562-1568,7.基金项目
国家自然基金(81374013)；湖南省卫计委科研课题(B2015-182)。@@@@This work was supported by the National Nature Scientific Foundation of China (81374013) （81374013）
the Scientific Research Foundation of Health and Family Planning Committee of Hunan Province (B2015-182), P. R. China （B2015-182）
