湖南农业大学学报（自然科学版）2016，Vol.42Issue(5)：500-504,5.
花粉管通道法介导山葡萄VaCBF3基因转化杨树的初步研究
Preliminary study on transgenic poplar mediated with VaCBF3 gene from Vitis amurensis by pollen-tube pathway approach
摘要
Abstract
By taking crossing offspringof Populus deltoides‘D189’ andP. deltoidesBartr. el. ‘Lux’×P. deltoides Bartr ‘Shanhaiguan’ as parents, the plasmid carried withVitisCBF3 (VaCBF3) was transferred into poplar through stigma-drop by Pollen-Tube Pathway approach. The results from PCR–analysis showed that 5 out of 52 transformed offspring were amplified in the form of target stripe, which indicated that the target gene had been integrated into poplar genome. The result could provide a clue for further research on new cold-resistance poplar via pollen-tube pathway approach.关键词
山葡萄VaCBF3基因/美洲黑杨‘D189’/辽宁杨/花粉管通道法/遗传转化Key words
VaCBF3 gene/Populus deltoides‘D189’/P. deltoides Bartr.el.‘Lux’×P. deltoidesBartr. ‘Shanhaiguan’/Pollen-Tube Pathway/genetic transformation分类
农业科技引用本文复制引用
冯连荣,王占斌,尹杰,宋立志,彭儒胜,赵鑫闻..花粉管通道法介导山葡萄VaCBF3基因转化杨树的初步研究[J].湖南农业大学学报（自然科学版）,2016,42(5):500-504,5.基金项目
辽宁省科学事业公益研究基因项目(2014002016) （2014002016）
