湖南农业大学学报（自然科学版）2016，Vol.42Issue(5)：505-510,6.
猪胰岛素诱导1基因的cDNA克隆和差异表达及蛋白质序列分析
Cloning and differential expression of insulin induced gene 1 in pig and the analysis on its protein sequence
摘要
Abstract
The study aims to clone the pig insulin induced gene 1(INSIG1) and conducts its protein sequences analysis. According to conserved sequence geneINSIG1 in human, a pair of primers was designed. By adopting pig total RNA as template, theINSIG1 gene sequences was cloned using RT–PCR, then theINSIG1 gene was connected to pMD 19–T Simple vector. The positive clone was sequenced after the identification of clone by PCR, and the sequence characterization was performed from biology information analysis software. The results from relative real-time PCR analysis indicated thatINSIG1 transcript abundance was the highest in lung, next in liver, spleen, and heart in turn. The experiment obtained a 999 bp sequence, of which included 831 bp open reading frame encoded with 276 amino acids. From the results of bioinformatics analysis showed that the protein does not contain signal peptide sequence, and from the results of amino acid sequence alignment showed that pigINSIG1 had high similarity score (more than 71%) with other animals in GenBank.关键词
猪/胰岛素诱导1基因/基因克隆/序列分析Key words
pig/insulin induced gene 1(INSIG1)/gene clone/sequence analysis分类
农业科技引用本文复制引用
王京京,魏麟,陈斌..猪胰岛素诱导1基因的cDNA克隆和差异表达及蛋白质序列分析[J].湖南农业大学学报（自然科学版）,2016,42(5):505-510,6.基金项目
国家现代农业产业技术体系建设专项(CARS-36)；湖南农业大学青年科学基金项目(14QN27) （CARS-36）
