江苏大学学报（医学版）2016，Vol.26Issue(5)：387-390,4.DOI:10.13312/j.issn.1671-7783.y160063
大麻素受体1拮抗剂ZH-101-S对糖尿病大鼠肾脏的保护作用
Protective effect of novel CB1 receptor antagonist ZH-101-S on renal function in diabetic rats
摘要
Abstract
Objective:To investigate the protective effect of novel cannabinoid(CB)1 receptor antago-nist ZH-1 01-S on kidneys in streptozotocin induced diabetic rats.Methods:Diabetic models were estab-lished by intraperitoneal injection of streptozotocin,rats were randomized into diabetes mellitus group(DM group,normal saline,i.p.),low dose group(0.3 mg/kg ZH-1 01-S,i.p.),middle dose group(1 .0 mg/kg ZH-1 01-S,i.p.),and high dose group(3.0 mg/kg ZH-1 01-S,i.p.);and healthy rats were selected as control group(normal saline,i.p.).After 8 weeks,the serum urea nitrogen(BUN),serum creatinine (Scr),urine albumin excretion rate(UAER),and the level of malondialdehyde(MDA)and the activity of superoxide dismutase(SOD)in renal tissues were detected;the pathological changes of renal tissues were observed by HE staining;the expression of voltage-dependent anion channel(VDAC)protein in renal tis-sues were examined by Western blotting.Results:Compared with control group,DM group showed higher levels of UAER,Scr and BUN(P<0.05 ),decreased SOD activity(P<0.01 ),and increased expression of MDA(P<0.05);decreased VDAC expression(P<0.01 );glomerular volume increased,renal tubular epithelial cell edema,mesangial matrix increased.Compared with DM group,high dose group had lower levels of UAER,Scr and BUN,increased SOD activity,lower content of malondialdehyde;increased VDAC expression(P<0.01 ),the pathological morphology of the kidney was improved.Conclusion:ZH-1 01-S showed protective effect on the kidney of diabetic rats,which may be related to the inhibition of oxi-dative stress and the improvement of kidney mitochondrial function.关键词
大麻素受体/拮抗剂/糖尿病肾病/氧化应激/电压依赖性离子通道Key words
cannabinoid1receptor/antagonist/diabeticnephropathy/oxidativestress/voltage-de-pendent anion channel分类
医药卫生引用本文复制引用
邹昭,吴晨光,姜惠,高静,范如霖,王丽,张敏..大麻素受体1拮抗剂ZH-101-S对糖尿病大鼠肾脏的保护作用[J].江苏大学学报（医学版）,2016,26(5):387-390,4.基金项目
镇江市社会发展项目 （）
