江苏大学学报（医学版）2016，Vol.26Issue(5)：391-394,4.DOI:10.13312/j.issn.1671-7783.y160205
人孕酮受体膜组分1表达载体的构建及其重组蛋白对体外血管形成的诱导作用
Construction of recombinant plasmid containing human progesterone receptor membrane component 1 gene and effect of the corresponding protein product on angiogenesis in vitro
摘要
Abstract
Objective:To construct the recombinant plasmid containing progesterone receptor membrane component 1 (PGRMC)gene and investigate the effect of the recombinant protein product on angiogenesis by ovarian cancer cells.Methods:We constructed the recombinant plasmid pST-PGRMC1 from ovarian cancer cells by PCR.The pST-PGRMC1 plasmid was transfected to HEK293T cells and SKOV-3 cells,re-spectively.The protein level of PGRMC1 was detected by western blotting.The mRNA and protein level of VEGF was detected by real-time PCR and ELISA.And then,we investigated the effect of PGRMC1 on an-giogenesis by ovarian cancer cells in vitro.Results:The recombinant pST-PGRMC1 plasmid was constructed successfully.The recombinant protein could be detected in HEK293T cells and SKOV-3 cells by western blotting.The overexpression of PGRMC1 promoted the synthesis of VEGF and microtubule formation by SK-OV-3 in vitro.Conclusion:The recombinant protein PGRMC1 plays an important role in the microtubule formation of ovarian cancer cells in vitro,and promotes the metastasis of the ovarian cancer cells.关键词
人孕酮受体膜组分1/质粒构建/血管形成Key words
progesteronereceptormembranecomponent1/plasmidconstruction/angiogenesis分类
医药卫生引用本文复制引用
季明德,朱小飞,杨学文,张春兵..人孕酮受体膜组分1表达载体的构建及其重组蛋白对体外血管形成的诱导作用[J].江苏大学学报（医学版）,2016,26(5):391-394,4.基金项目
国家自然科学基金资助项目 （）
