江苏大学学报（医学版）2016，Vol.26Issue(5)：400-404,5.DOI:10.13312/j.issn.1671-7783.y160214
Bcl-3在人乳腺癌组织中的表达及对乳腺癌MDA-MB-231细胞增殖与凋亡的影响
Expression of Bcl-3 in breast cancer tissues and its effect on the proliferation and apoptosis of human breast cancer cell line MDA-MB-231
袁建良 1胡永伟 1丁厚中1
作者信息
- 1. 江苏大学附属昆山医院普外科，江苏 昆山215300
- 折叠
摘要
Abstract
Objective:To explore Bcl-3 expression in human breast cancer and its effect on breast cell proliferation and apoptosis.Methods:Bcl-3 expression in 80 breast cancer tissues with corresponding adja-cent tissues were detected by immunohistochemical staining.MDA-MB-231 cells were randomized into blank control group(routine culture),negative control group(transfected with control siRNA)and Bcl-3 siRNA group(transfected with Bcl-3 siRNA);Bcl-3 expression was detected by western blotting.MTT as-say,plate clone formation assay and flow cytometry were used to determine the cell proliferation,cloning formation efficiency and apoptosis rate,respectively.Results:The positive rate of Bcl-3 expression in breast cancer tissue was 72.5%,while in adjacent tissues was 51 .3%.The Bcl-3 expression in breast cancer tissues was positively correlated with histological grade,lymph node metastasis and Her-2 expression (P<0.05 ).Bcl-3 expression in blank control group and negative control group was significantly higher than in Bcl-3 siRNA group(P<0.01 ).Compared with blank control group and negative control group,Bcl-3 siRNA group showed lower cell proliferation rate,lower colony forming efficiency,higher apoptosis rate (P<0.01 ).Conclusion:Bcl-3 expression in breast cancer tissues was high,and silencing it could suppress the growth of MDA-MB-231 breast cancer cells by reducing cell proliferation,inhibiting cloning formation,and inducing cell apoptosis.关键词
Bcl-3/乳腺癌/增殖/凋亡Key words
Bcl-3/breastcancer/proliferation/apoptosis分类
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袁建良,胡永伟,丁厚中..Bcl-3在人乳腺癌组织中的表达及对乳腺癌MDA-MB-231细胞增殖与凋亡的影响[J].江苏大学学报（医学版）,2016,26(5):400-404,5.
