军事医学2016，Vol.40Issue(10)：801-804,808,5.DOI:10.7644/j.issn.1674-9960.2016.10.006
PRDX3真核表达载体构建及亚细胞定位
Construction and identification of PRDX3 eukaryotic expression vector
摘要
Abstract
Objective To construct the eukaryotic expression vector of PRDX3 labeled with FLAG tag and to study its localization in human tongue cancer cell line SCC15.Methods PRDX3 gene was obtained from the breast library by PCR and cloned into PCDH vector to construct PCDH-FLAG-PRDX3.The plasmid was transiently transfected into 293T cells and the expression was detected by Western blot.Subcellular localization was detected by cellular immunofluorescence.Results The result of double digestion and sequencing showed that PCDH-FLAG-PRDX3 eukaryotic expression vector was constructed.The expression of FLAG-PRDX3 in human 293T cells was positively confirmed by Western blotting.In human tongue cancer cell line SCC15, the result of cellular immunofluorescence showed FLAG-PRDX3 was located in the cytoplasm rather than in the nucleus.Conclusion PRDX3 eukaryotic expression vector labeled with FLAG tag is constructed successfully, which is located in cytoplasm in human SCC15 cells.Construction and identification of PRDX3 could shed light on the function and mechanism of PRDX3 in tongue cancer.关键词
过氧化物还原酶3/克隆/真核表达/亚细胞定位Key words
peroxiredoxin3/clone/eukaryotic expression/subcellular localization分类
医药卫生引用本文复制引用
张春霞,程龙,麦宏旭,王琳,张菊会,王恩群,叶棋浓..PRDX3真核表达载体构建及亚细胞定位[J].军事医学,2016,40(10):801-804,808,5.基金项目
国家自然科学基金面上资助项目 （）
