军事医学2016，Vol.40Issue(10)：814-818,5.DOI:10.7644/j.issn.1674-9960.2016.10.009
3株木糖氧化无色杆菌烈性噬菌体的最佳保存方法研究
Optimal condition for preservation of three Achromobacter xylosoxidans bacteriophages
摘要
Abstract
Objective To find simple and effective methods of preservation for Achromobacter xylosoxidans bacteriophages.Methods The plaque forming unit( PFU) of surviving phages under different preservation conditions and temperatures at different time points was determined.Results and Conclusion The titers of phiAxp-1 and phiAxp-2 main-tained at the initial 1010 PFU/ml, and that of phiAxp-3 decreased slightly from 1011 PFU/ml to 1010 PFU/ml 16 months after the three Achromobacter xylosoxidans bacteriophages were stored in glycerol and dimethyl sulfoxide at -80℃, -20℃and 4℃.When stored in chloroform at 4℃for 16 months, the titers of all the three phages decreased slightly but were higher than at other temperatures (-80℃, -20℃, room temperature, and 37℃) .Thus these methods can effectively preserve Achromobacter xylosoxidans bacteriophages.关键词
木糖氧化无色杆菌/噬菌体/保存Key words
Achromobacter xylosoxidans/bacteriophage/preservation分类
生物科学引用本文复制引用
黎尔纳,马艳艳,程伟伟,蒋爱民,袁静,赵向娜..3株木糖氧化无色杆菌烈性噬菌体的最佳保存方法研究[J].军事医学,2016,40(10):814-818,5.基金项目
国家自然科学基金资助项目 （）
