江西农业大学学报2016，Vol.38Issue(5)：866-870,5.DOI:10.13836/j.jjau.2016122
江西胜红蓟黄脉病病原的分子鉴定
Molecular Identification of the Pathogen Inducing Ageratum Yellow Vein Disease in Jiangxi
摘要
Abstract
To determine the virus species infecting Ageratum conyzoides in Ganzhou, Jiangxi Province, weed samples showing typical geminivirus⁃like symptoms were tested by PCR technique.A fragment of 500 bp was consistently amplified from total DNA extracts of 3 samples with a pair of specific primer.Comparison of se⁃quences of the 500 bp fragment revealed that these samples were infected by a same virus and thus an isolate denoted JX01 was selected for further sequence analysis.The complete sequence of JX01 comprised 2 754 nu⁃cleotides with typical genomic organization of geminiviral DNA⁃A.JX01 was most closely related to Papaya leaf curl China virus isolate Fz10 ( PaLCCNV⁃Fz10) ,with 99.7% nucleotide sequence identity.By using the specific primers for the full length of betasatellite,a fragment of about 1 300 bp was amplified.The complete sequence of betasatellite (JX⁃Gz01β) was determined to be 1 314 nucleotides.Sequence analysis showed that JX⁃Gz01βwas most closely related to Ageratum yellow vein China betasatellite isolate Hn9 (AYVCNB⁃Hn9),with 94.3% nu⁃cleotide sequence identity.Phylogenetic analysis showed that JX01 clustered together with isolates of PaLCCNV. This is the first report of PaLCCNV infecting Ageratum conyzoides in Jiangxi Province.关键词
双生病毒/中国番木瓜曲叶病毒/胜红蓟/中国胜红蓟黄脉病毒Key words
Geminivirus/Papaya leaf curl China virus/Ageratum conyzoides/Ageratum yellow vein China virus分类
农业科技引用本文复制引用
王禄,林文武,欧阳智刚,宛柏杰,吴祖建,张洁..江西胜红蓟黄脉病病原的分子鉴定[J].江西农业大学学报,2016,38(5):866-870,5.基金项目
国家自然科学基金项目（31301640）、福建省教育厅科研项目（ JA13103）和福建农林大学A类毕业生科研启动基金（132130011） Project supported by the National Natural Science Foundation of China （31301640），the project of Department of Education of Fujian Province （ JA13103） and the Scientific Research Foundation of Fujian Agriculture and For-estry University （）
