农业工程学报2016，Vol.32Issue(z2)：69-76,8.DOI:10.11975/j.issn.1002-6819.2016.z2.010
稻田水分管理和秸秆还田对甲烷排放的微生物影响
Methane-related mierobe influenced by water management and rile straw returning in paddy soil
摘要
Abstract
In order to investigate the mechanism of methane emission based on different water management and rice straw returning, this study determined the CH4 flux and the abundance and community structure of methanogens and methane oxidizing bacteria. Quantitative polymerase chain reaction (qPCR) and terminal restriction fragment length polymorphism (T-RFLP) were used to determine the abundance and community composition ofmcrA-containing methanogens and pmoA-containing methane oxidizing bacteria in DNA and cDNA level from paddy field. The soil samples taken from the paddy soil at Changsha Research Station for Agricultural & Environmental Monitoring of Institute of Subtropical Agricultural, the Chinese Academy of Sciences. Four treatments included the following: CF (balanced fertilizer without rice straw returning and long-term flooding), HS+CF (balanced fertilizer with 6.0 t/hm2 rice straw returning and long-term flooding), IF (balanced fertilizer without rice straw returning and intermittent irrigation), HS+IF (balanced fertilizer with 6.0 t/hm2 rice straw returning and intermittent irrigation). The results showed that long-term flooding significantly increased methane emissions, but not rice straw returning. And there was no significant interaction between the treatments of water management and rice straw returning. The community structure of methanogens and methane oxidizing bacteria were affected by water management, but not by rice straw returning based on cluster analysis. At the level of cDNA, the community structure of methanogens and methane oxidizing bacteria were different between the treatments of long-term flooding and intermittent irrigation under the condition with or without rice straw returning. While, at the level of DNA, the communities structure were only affected by water management under the condition without water management. At the T-RFLP profile, there were obviously difference between the community structure at DNA level and transcriptional community structure at cDNA level of methanogens and methane oxidizing bacteria, which were more sensitive to water management at cDNA level than that at DNA level. The abundance of methanogens were affected by water management and rice straw returning at cDNA and DNA level, but not of methane oxidizing bacteria. CH4 emissions significantly correlated with the ratio of the abundance ofmcrA andpmoA genes at DNA level, not with the abundance ofmcrA orpmoA genes. So the CH4 emissions determined by the ratio of the abundance of methanogens and methane oxidizing bacteria, not of methanogens or methane oxidizing bacteria. In totally, the community structure and abundance of methanogens and methane oxidizing bacteria is more affected by water management than rice straw returning based on the short-tern experiment. The community structure and abundance of methanogens and methane oxidizing bacteria are affected by water management at DNA and cDNA level. The ratio of the abundance of mcrA andpmoA genes is the key to control the methane emissions from paddy field.关键词
水分管理/秸秆/微生物/甲烷氧化菌/群落结构/DNA/cDNAKey words
water management/straw/microbiology/methane oxidizing bacteria/community structure/DNA/cDNA分类
资源环境引用本文复制引用
吴讷,侯海军,汤亚芳,沈建林,刘波,魏文学,秦红灵..稻田水分管理和秸秆还田对甲烷排放的微生物影响[J].农业工程学报,2016,32(z2):69-76,8.基金项目
国家科技支撑项目（2012BAD14B03）；国家“973”计划项目（2012CB417106）；国家自然科学基金面上项目 （）
