山东医药2016，Vol.56Issue(41)：6-9,4.DOI:10.3969/j.issn.1002-266X.2016.41.002
EBLN2基因对肝癌细胞增殖的抑制作用及其机制
Inhibitory effect of EBLN2 gene on proIiferation of hepatocellular carcinoma cells
摘要
Abstract
Objective To study the inhibitory effects of endogenous Bornavirus-like nucleoprotein 2 (EBLN2)gene on proliferation of hepatocellular carcinoma (HCC)cell lines and its mechimasm.Methods The real-time fluorescent quantitative RT-PCR and Western blotting was used to detect the expression of EBLN2 in HCC cell lines (HepG2,SK-hep-1,Huh7,Hep3B,MHCC-97H,PLC /PRF /5,Focus,SMMC7721,YY-8103 and LO2)and human HCC tissue samples. The overexpression of EBLN2 was carried out in Focus and YY-8103 cells with low expression of EBLN2.The recombinant plasmid pCMV-EBLN2 was transfected into Focus and YY-8103 cells.At 24 h,the effects of overexpression of EBLN2 gene on the proliferation of HCC cell lines,plate clone formation ability,soft agar clone formation ability,and cell cycle.Re-sults The quantitative and semi-quantitative PCR results showed that EBLN2 mRNA expression was down-regulated in 59% HCC samples.Further,compared with the normal hepatic tissues,EBLN2 expression was down-regulated in cell lines such as HepG2,SK-hep-1,Huh7,Hep3B,MHCC-97H,PLC /PRF /5,Focus,SMMC7721,YY-8103 and LO2 with the percentage of 48%,73%,72%,79%,84%,72%,87%,74%,82% and 80%,respectively (all P <0.05).After the Pcmv-EBLN2 plasmid was transferred into cell lines YY-8103 and Focus for 24 h,the proliferation rates of YY-8103 and Focus cell lines were 72.2% and 85%.The plate clone formation rates of YY-8103 and Focus cells were 52.6% and 25.6%,and the soft agar clone formation rates of YY-8103 and Focus cells were 40.9% and 37.5%,and significant difference was found,all P <0.05.The cell cycle transition from G1 phase to S phase was inhibited in the YY-8103 and Focus cells.Conclusions EBLN2 is down-regulated in HCC tissues and inhibits the proliferation of HCC cells by regula-ting the cell cycle.关键词
肝细胞癌/人类内源性非逆转录博纳病毒样核蛋白序列2基因/抑癌基因/细胞增殖Key words
hepatocellular carcinoma/endogenous Bornavirus-like nucleoprotein 2/tumor suppressor gene/cell pro-liferation分类
医药卫生引用本文复制引用
魏岩冰,韩泽广..EBLN2基因对肝癌细胞增殖的抑制作用及其机制[J].山东医药,2016,56(41):6-9,4.基金项目
国家科技重大专项课题（2012zx10001012）。 （）
