山东医药2016，Vol.56Issue(41)：14-17,4.DOI:10.3969/j.issn.1002-266X.2016.41.004
白花九里明对急性肝损伤小鼠的保护作用及机制
Protective effect of BIumea megacephaIa on acute Iiver injury in mice
摘要
Abstract
Objective To investigate the protective effect of Blumea megacephala extracting solution on acute liver injury of mice and its mechanism of action.Methods Fifty KMmice were randomly divided into 5 groups.Normal con-trol group and model control group were given 0.9% NaCl injection,bifendatatum group was given 0.2 g/kg bifendatatum, Blumea megacephala low-concentration group and high-concentration group were respectively given 15 and 30 g/kg Blumea megacephala extracting solution for 7 days by gastric irrigation.Meanwhile,on day 1,3 and 5 of gastric irrigation,the nor-mal control group was given 0.9% NaCl by intraperitoneal injection,the rest of the groups were given 0.1%Carbon tetra-chloride (CCL4 )liquid.One hour 1 h after the last intragastric administration,we collected and separated the serum,and took the left lobe of liver to make and separate the liver supernatant,took the right lobe of liver to make tissue section.The total protein (TP),glutamic-pyruvic transaminase (GPT),glutamic-oxalacetic transaminase (GOT),superoxide dis-mutase (SOD)and malondialdehyde (MDA)content or activity in liver homogenate and serum were detected and we ob-served the structure changes of liver tissue microscopically.Results The TP content of liver homogenate in the model con-trol group,bifendatatum group and Blumea megacephala low-concentration group was obviously higher than that of the nor-mal control group (all P <0.01).The TP content of liver homogenate in the Blumea megacephala high-concentration group was higher than that of the normal control group and model control group (all P <0.05).The GPT activity of liver homoge-nate in the normal control group,model control group and Blumea megacephala low-concentration group was higher than that of the bifendatatum group and Blumea megacephala high-concentration group (all P <0.05).The GOT activity of ser-um in the Blumea megacephala low-concentration group was lower than that of the normal control group (P <0.05).The SOD activity of liver homogenate in the Blumea megacephala high-concentration group,bifendatatum group and model con-trol group was lower than that of the normal control group (all P <0.05).The SOD activity of liver homogenate in the Blumea megacephala low-concentration group was higher than that of the bifendatatum group (P <0.05),but the SOD ac-tivity of serum was higher than that of the normal control group,model control group,bifendatatum group and Blumea megacephala high-concentration group (P <0.01).The MDA content of liver homogenate in the Blumea megacephala low-concentration group and high-concentration group was lower than that of the normal control group (all P <0.05).No statis-tically significant difference were found in the TP content,GPT activity,GOT activity,SOD activity and MDA content of serum among these groups (P >0.05).The histological examinations showed that the liver tissue structure was clear,less liver cells were degenerated,dissolved and necrotic and a small number of central venous cavities had a small amount of shedding liver tissue structure in the bifendatatum group,Blumea megacephala low-concentration and high-concentration group as compared with the model control group.Conclusions Blumea megacephala have protective effect on CCl4-in-duced acute liver injury with dose-effect relationship.The high-concentration Blumea megacephala have the same effect as bifendatatum on liver cells,whose mechanism may be related to facilitation and regulation of SOD in scavenging free radi-cals,blocking and reducing lipid peroxidation of liver cells,and enhancing protein synthesis in liver.关键词
急性肝损伤/壮药/白花九里明提取液/谷丙转氨酶活性/谷草转氨酶活性/超氧化物歧化酶活性/丙二醛/小鼠Key words
acute liver injury/Zhuang-nationality drugs/Blumea megacephala extracting solution/glutamic-pyruvic transaminase activity/glutamic-oxalacetic transaminase activity/superoxide dismutase activity/malondialdehyde/mice分类
医药卫生引用本文复制引用
刘桂彪,方晓燕,张铭锋,申璐,黄剑秋,郭莹,黄文飞,闭丽华,王保山,王彩冰..白花九里明对急性肝损伤小鼠的保护作用及机制[J].山东医药,2016,56(41):14-17,4.基金项目
2015年地方高校国家级大学生创新创业训练计划项目（201510599009）。 （）
