山东医药2016，Vol.56Issue(41)：22-25,4.DOI:10.3969/j.issn.1002-266X.2016.41.006
幽门螺旋杆菌和 p14ARF 在蒙古族、汉族胃癌患者癌组织中的表达变化及意义
Expression changes of Hp and p14 ARF of patients with gastric carcinoma in Han and Mongolian nationalities
摘要
Abstract
Objective To detect the expression of Helicobacter pylori (Hp)and p14ARF in the gastric cancer tissues, paracancerous tissue and normal tissues of patients with gastric carcinoma in Han and Mongolian nationalities and the mech-anism of two factors in the occurrence of gastric cancer.Methods Immunohistochemical (S-P)method,a modified War-thin-Starry (W-S)silver staining method and reverse transcription polymerase chain reaction (RT-PCR)were used to de-tect the expression of protein and mRNA of Hp and p14ARF in gastric cancer tissues,paracancerous tissues and normal tis-sues from 27 cases of Mongolian patients and 30 cases of Han patients with gastric cancer.Spearman analysis was used to analyze the correlation between the Hp and p14ARF expression in the gastric cancer tissues of patients from two nationalities. Results The expression of p14ARF in gastric cancer tissues of Mongolian and Han patients was lower than that in paracan-cerous and normal tissues,and the difference was statistically significant (all P <0.05).The expression of p14ARF in the paracancerous tissues was lower than that in the normal tissues,and the difference was statistically significant (P <0.05), Meanwhile,the expression of p14ARF itself in two nationalities had no significant difference (P >0.05).The positive rate of Hp was high in the gastric cancer tissues of patients in Mongolian and Han nationalities,and there was no significant differ-ence in the rate of infection between the two nationalities (P >0.05).The relative expression of p14ARF mRNA was statisti-cally significant among the cancer tissues,paracancerous tissues and normal tissues of Han patients with gastric cancer as well as in Mongolian patients (all P <0.05).There was no significant difference in the relative expression of p14ARF mR-NA of cancer tissues,paracancerous tissues and normal tissues of Mongolian and Han patients (P >0.05).Spearman cor-relation analysis showed that the positive expression of Hp and p14ARF was negatively correlated with each other in Han and Mongolian gastric cancer tissues (r =-0.406,-0.433,all P <0.05).Conclusions The expression of p14ARF is low, the positive rate of Hp is high,and p14ARF and Hp is negatively correlated in cancer tissues of patients with gastric cancer. The pathogenic mechanism of Hp and p14ARF in the Mongolian and Han patients with gastric cancer may be the same,and both of Hp and p14ARF may jointly promote the occurrence of gastric cancer.关键词
胃癌/幽门螺旋杆菌/p14ARF基因/蒙古族/汉族Key words
gastric carcinoma/Helicobacter pylori/p14ARF gene/Mongolian nationality/Han nationality分类
医药卫生引用本文复制引用
鲁志刚,冷雪芹,李燕,贾慧琼..幽门螺旋杆菌和 p14ARF 在蒙古族、汉族胃癌患者癌组织中的表达变化及意义[J].山东医药,2016,56(41):22-25,4.基金项目
内蒙古自治区自然科学基金项目（2014MS08109）。 （）
