森林工程2016，Vol.32Issue(6)：10-14,5.
杂交杨转录因子Pdt-ERF3抗锈病基因表达
Pdt-ERF3 Expression of the Hybrid PoplarInfected with Melampsora Larici-populina
摘要
Abstract
Melampsora larici-populina strongly endangers a variety of poplars.In order to investigate the molecular mechanisms of transcriptional regulation factors ERF3 in the reaction of the poplar resisting M.larici-populina,ERF3 genes from Populus deltoides × P.trichocarpa (less susceptible to E4 M.larici-populina) and Populus × euramericana (very susceptible to E4.larici-populina) were cloned and named as Pdt-ERF3 and Pnd-ERF3,respectively.The expression quantity change of Pdt-ERF3 and Pnd-ERF3 of before and after vaccination E4 to strong pathogenic M.larici-populina.were analyzed by employing Q-PCR technique.The expression quantity of Pdt-ERF3 reached the peak at 12-hour post inoculation (hpi).The expression quantity of Pnd-ERF3 increased significantly at 24hpi and reached the peak at 96hpi.Compared to Pdt-ERF3,the peak expression quantity of Pnd-ERF3 was delayed 84 hours.Both expression quantities of the Pnd-ERF3 and Pdt-ERF3 appeared a significant decreasing except at 168hpi,and the expression quantities of Pnd-ERF3 and Pdt-ERF3 response to rust stress were different in multiple stages.It was predicted that the expression quantity of Pdt-ERF3 was subject to the regulation of MAPK6 according to homeotic gene of Arabidopsis thaliana.This study provided a theoretical foundation for further studying ERF gene and the possible use of these genes for the production of rust resistance poplar.关键词
转录调控因子/ERF/乙烯/落叶松-杨栅锈菌Key words
Transcriptional regulation factors/ERF/Ethylene/Melampsora larici-populina分类
农业科技引用本文复制引用
王峰,程诗评,牛春阳,李丹蕾,陈俏丽,张瑞芝,沈圣智..杂交杨转录因子Pdt-ERF3抗锈病基因表达[J].森林工程,2016,32(6):10-14,5.基金项目
国家自然科学基金项目(31500524) （31500524）
中央高校基本科研业务费专项资金项目(2572015EY02,2572015CA09,2572016CA04) （2572015EY02,2572015CA09,2572016CA04）
大学生创新性实验计划项目(201510225129) （201510225129）
