中国生态农业学报2016，Vol.24Issue(11)：1529-1538,10.DOI:10.13930/j.cnki.cjea.160421
低温胁迫下内源ABA、GA及比值对白菜型和甘蓝型冬油菜抗寒性的响应
Response of endogenous ABA and GA to cold resistance ofBrassica rapa L. andBrassica napus L.
摘要
Abstract
AbstractCold resistance is critical in winter rapeseed production in northern China. The abscisic acid (ABA) plant hormone, also known as “stress hormone” is an important signal molecule for the regulation of plant cold resistance. Study of possible regulatory mechanisms of endogenous ABA and GA is needed to develop a valuable insight into the mechanism of cold resistance both in winter rapeseedBrassica rapa L. andBrassica napus L. Thus we investigated the regulation of endogenous hormones in cold-resistance using eight winter rapeseed cultivars with different gradients of cold tolerance in a pot experiment. Different winter rapeseed cultivars were cultivated in a growth chamber with temperature conditions of 25℃, 10℃, 2℃ and-5℃, respectively. The endogenous ABA, GA and chlorophyll contents were measured. Regression analysis showed a significantly negative linear correlation between temperature and endogenous ABA content, with a regression equation ofy=-ax+ b. With decreasing of temperature, the endogenous ABA content increased slightly at 10℃ and then drastically at both 2℃ and-5℃. There were remarkable differences in ABA content among the temperature treatments and interaction of temperature and crop cultivar. The trend in endogenous GA content was the exact contrast of that of ABA, highlighting the antagonism between the both phytohormones. Temperature had no significant effect on endogenous ABA content for temperatures > 0℃ in different winter rapeseed cultivars. However, temperature effect on endogenous ABA content ofB. rapa was more significant than that of B.napus, and the cold tolerant cultivar more sensitive at a temperature of-5℃. The increase in ABA content contributed to the change in chlorophyll, which initially increased and then decreasing with decreasing temperature, and with an overall declining trend. The response mechanisms betweenB.rapa andB.napus were different, which resulted in lower chlorophyll content inB.rapa than inB.napus. The leaf wilting date ofB.rapa delayed well into wintering stage, with leaf wilting period of strongly cold resistant varieties occurring early to halt root nutrient uptake. Winter rapeseed root stored enough organic matter to increase its cold resistance and defend against low temperature injury. Thus with decreasing temperature, endogenous ABA content of winter rapeseed leaf increased. However, as chlorophyll degraded,B. rapa winter rapeseed leaf withered early, occurring in advance along with high ABA content after low temperature stress. This was the main reason and the stronger characteristic biological performance ofB.rapa over B.napus in terms of cold resistance.关键词
白菜型/甘蓝型/冬油菜/ABA/GA/叶绿素/低温胁迫/抗寒性Key words
Brassica rapa L./Brassica napus L./Winter rapeseed/Endogenous ABA/Endogenous GA/Chlorophyll/Low temperature stress/Cold resistance分类
农业科学引用本文复制引用
刘海卿,赵艳宁,董小云,曾秀存,许耀照,方园,武军艳,陈奇,孙万仓,刘自刚,方彦,米超,蒲媛媛..低温胁迫下内源ABA、GA及比值对白菜型和甘蓝型冬油菜抗寒性的响应[J].中国生态农业学报,2016,24(11):1529-1538,10.基金项目
国家自然科学基金项目(31460356,31560397,31660404)、农业部产业技术体系项目(CARS-13)、国家重点基础研究发展计划(973计划)项目(2015CB150206)、国家农业科技成果转化项目(2014G10000317)、“油菜杂种优势利用技术与强优势杂交种创制”项目(2016YFD0101300)和甘肃省自然科学基金项目(145RJZG050,1506RJZG051)资助* Funded by the National Natural Science Foundation of China (31460356,31560397,31660404), the China Agriculture Research System (CARS-13), the National Basic Research Program of China (973 Program)(2015CB150206), the National Agricultural Science and Technology Achievements Transformation Projects (2014G10000317),‘the Rapeseed Heterosis Utilization Technology and Strong Advantage Creation of Hybrid’(2016YFD0101300), the Natural Science Foundation of Gansu (145RJZG050,1506RJZG051) （31460356,31560397,31660404）
