生物技术通报2016，Vol.32Issue(11)：72-79,8.DOI:10.13560/j.cnki.biotech.bull.1985.2016.11.010
基因侧翼序列扩增技术的应用进展
Application Progress on Amplified Methods to Clone Flanking Sequence
蒲远波 1郭翠 2平淑珍2
作者信息
- 1. 川北幼儿师范高等专科学校，广元 628017
- 2. 中国农业科学院生物技术研究所，北京 100081
- 折叠
摘要
Abstract
Amplifying the flanking sequence of genes plays an important role in molecular biology research. Until so far,the methods used to clone flanking sequence can been categorized into three groups :I-PCR,LA-PCR and TAIL-PCR. This paper briefly introduces the principle and present applications of these methods,which provides basis for researchers to choose more reliable and reasonable method.关键词
侧翼序列/反向 PCR/外源接头介导 PCR/半随机引物 PCRKey words
flanking sequence/I-PCR/LA-PCR/TAIL-PCR引用本文复制引用
蒲远波,郭翠,平淑珍..基因侧翼序列扩增技术的应用进展[J].生物技术通报,2016,32(11):72-79,8.
