生物技术通报2016，Vol.32Issue(11)：93-98,6.DOI:10.13560/j.cnki.biotech.bull.1985.2016.11.012
端粒长度检测方法及其应用
Methods of Measuring Telomere Length and Its Application
摘要
Abstract
Aging and tumor are the two main factors influencing human longevity. Many studies have proved that telomere play important roles in these progresses. Thus, it is critical to familiarize with assay methods of telomere length. The most commonly used methods include telomere restriction fragment(TRF)length analysis, quantitative PCR(qPCR), fluorescent in situ hybridization(FISH)and single telomere length analysis(STELA). However, each method has the scope of application, in the process of research and application, we have to understand the principle of each method, its advantages and disadvantages, then we may choose the appropriate detection method to achieve the aim of our study and solve specific issues. The commonly used detection methods of telomere length are summarized here.关键词
端粒长度/端粒末端限制性片段分析/定量 PCR/荧光原位杂交/单链端粒长度分析Key words
telomere length/TRF/qPCR/FISH/STELA引用本文复制引用
张彬,王长利..端粒长度检测方法及其应用[J].生物技术通报,2016,32(11):93-98,6.基金项目
天津市抗癌重大专项 （）
