生物技术通报2016，Vol.32Issue(11)：152-161,10.DOI:10.13560/j.cnki.biotech.bull.1985.2016.11.018
花粉介导法获得耐草甘膦玉米植株及其耐性研究
Development of Transgenic Maize Plants Tolerant to Glyphosate via Pollen-mediated Transformation and Their Glyphosate Tolerance
摘要
Abstract
In order to develop transgenic maize plants tolerant to glyphosate and further study their herbicide tolerance and the genetic characteristics of exogenous gene in transgenic plants,plasmid pBI101-aroA-M12 harboring aroA-M12 gene was transformed into maize inbred Chang‘7-2’plants by pollen-mediated transformation method. Fifty-four transgenic plants were obtained by PCR detection and Southern blot analysis. The detection results of molecular analysis and bioassay in the field showed that target aroA-M12 gene was integrated into the genome of transgenic plants and could stably inherit to next generation. Moreover,the aroA-M12 gene conferred the glyphosate-tolerance to transgenic plants. The result of genetic rule of target gene in transgenic plants suggested that the target gene was segregated according to the ratio of 3∶1 in T2 generation transgenic plants(lines)and it was consistent with the Mendel law of single-factor heredity. The results of ELISA analysis and protein concentration determination of transgenic plants confirmed that the target gene was expressed in transgenic plants,and the protein amount of gene expression were 40.5-112.6 ng/g leaf fresh weight,there was a significant correlation between protein amount of expression of each transgenic plant and corresponding glyphosate-tolerance of that plant,correlation coefficients r = 0.942 3(P<0.01). Ultimately,4 pure lines of transgene with high glyphosate-tolerance were obtained from this experiment.关键词
玉米/aroA-M12 基因/草甘膦耐性/花粉介导/遗传分析Key words
maize/aroA-M12 gene/glyphosate-tolerance/pollen-mediated transformation/genetic analysis引用本文复制引用
杨慧珍,任志强,肖建红,卜华虎,刘惠民..花粉介导法获得耐草甘膦玉米植株及其耐性研究[J].生物技术通报,2016,32(11):152-161,10.基金项目
国家转基因生物新品种培育重大专项（2014ZX08003001-002-003，2016ZX08003001-002-003），山西省科技攻关项目 （）
