天津中医药2016，Vol.33Issue(11)：675-681,7.DOI:10.11656/j.issn.1672-1519.2016.11.11
和颜坤泰胶囊对卵巢去势大鼠子宫形态及细胞凋亡的影响
Anti-apoptotic and uterotrophic effects of Heyan Kuntai capsules in uterus of ovariectomized rats
摘要
Abstract
Objective] To investigate the effects of Heyan Kuntai capsules on uterus morphology and apoptosis in ovariectomized rats. [Methods] Female SD rats underwent ovariectomize surgery and were divided into model group, progynova group, Remifemin group and Heyan Kuntai capsule of high, medium and low dose groups (8, 4 and 2 g crude drug/kg respectively). The drugs were administered for 8 weeks. This article observed the impacts of drugs on uterus pathology, staining and apoptosis, detected the expression levels of Bcl-2 and Bax mRNAs and proteins. [Results] It was obtained significantly decreased epithelial thickness and reduced myometrium and endometrium cross-sectional area and wall thickness in the model group compared with the control and Heyan Kuntai capsule treated groups(P<0.01 or P<0.05). It was also decreased Bcl-2 and increased Bax mRNAs and proteins expressions (P<0.01 or P<0.05) in the model group. Further more, the model group showed increased apoptosis rate(P<0.01 or P<0.05) and reduced number of glands(P<0.01 or P<0.05) in the uterus. [Conclusion] Heyan Kuntai capsules elicits uterotrophic effect and attenuates apoptosis in the uterus cells by regulating mRNAs and protein expressions of Bcl-2 and Bax.关键词
和颜坤泰胶囊/子宫形态学/细胞凋亡/Bcl-2 mRNA和蛋白/Bax mRNA和蛋白Key words
Heyan Kuntai capsule/uterus morphology/apoptosis/Bcl-2 mRNA and protein/Bax mRNA and protein分类
医药卫生引用本文复制引用
黄春丽,张德芹,张青霞,文静..和颜坤泰胶囊对卵巢去势大鼠子宫形态及细胞凋亡的影响[J].天津中医药,2016,33(11):675-681,7.基金项目
国家重大新药创制科技重大专项（2012ZX09304007）。 （）
