天津中医药2016，Vol.33Issue(11)：689-693,5.DOI:10.11656/j.issn.1672-1519.2016.11.14
基于Ussing Chamber技术的红景天苷大鼠肠黏膜透过性研究
Study on permeabilities of Salidroside via rat mesentery mucosa by using Ussing Chamber
摘要
Abstract
Objective] To investigate the permeability of salidroside in mesentery mucosa of rat ex vivo. [Methods] The Ussing Chamber was employed to investigate the ex vivo permeability in rat mesentery mucosa of salidroside with different concentrations or with the addition of verapamil and SDS. [Results] It was found that the Flux of salidroside was increased with the increasing concentration, and there was no difference(P<0.05) in the apparent permeability coefficient (Papp) in different mesentery segments of rat. The Papp of salidroside got significantly increased in ileum and colon (P<0.01 or P<0.05), and reached to 2.76-fold compared with the control in ileum, when a certain amount of SDS was added in the donator side. [Conclusion] Salidroside could be slightly absorbed in all mesentery segments of rat with a passive concentration-dependent transportation. There were no effects of verapamil on permeation of salidroside, and SDS could significantly increase salidroside permeation in ileum and colon of rat.关键词
红景天苷/Ussing Chamber技术/P-糖蛋白/紧密连接/维拉帕米/十二烷基硫酸钠Key words
salidroside/Ussing Chamber/P-gp/tight junction/verapamil/SDS分类
医药卫生引用本文复制引用
顾星,姚少姿,皮佳鑫,刘博缨,韩煦,刘志东..基于Ussing Chamber技术的红景天苷大鼠肠黏膜透过性研究[J].天津中医药,2016,33(11):689-693,5.基金项目
新世纪优秀人才支持计划（NCET－12－1068）。 （）
